LOCUS       BBa_S04473              2625 bp    DNA     linear       26-Jun-2024
DEFINITION  E5500:K199173
ACCESSION   BBa_S04473
VERSION     BBa_S04473.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..741
                     /label=GFP protein
     CDS             22..741
                     /label=BBa_E0040
     misc_feature    1..741
                     /label=BBa_E5500
     misc_feature    754..757
                     /label=conserved
     RBS             750..761
                     /label=BBa_B0034
     CDS             768..1442
                     /label=mrfp1
     misc_feature    1449..1473
                     /label=Help:Barcodes
     CDS             768..1473
                     /label=BBa_E1010
     misc_feature    750..1473
                     /label=BBa_S04419
     CDS             1482..1569
                     /label=end of LacI coding region (inactive)
     misc_feature    1482..1681
                     /label=BBa_R0010
     misc_feature    1570..1607
                     /label=CAP binding site
     promoter        1618..1623
                     /label=-35
     promoter        1642..1647
                     /label=-10
     misc_feature    1647..1681
                     /label=LacI binding site
     misc_feature    1654..1654
                     /label=start
     promoter        1482..1681
                     /label=BBa_R0010
     misc_feature    1694..1697
                     /label=conserved
     RBS             1690..1701
                     /label=BBa_B0034
     misc_feature    1708..1709
                     /label=prefix
     misc_feature    1881..1881
                     /label=T
     misc_feature    2199..2199
                     /label=A
     CDS             1708..2457
                     /label=luxR
     misc_feature    1708..2463
                     /label=BBa_C0062
     misc_feature    2464..2488
                     /label=Help:Barcodes
     CDS             1708..2463
                     /label=BBa_C0062
     stem_loop       2508..2551
                     /label=stem_loop
     misc_feature    2497..2576
                     /label=BBa_B0010
     terminator      2497..2576
                     /label=BBa_B0010
     stem_loop       2592..2611
                     /label=T7 TE
     misc_feature    2618..2618
                     /label=stop
     polyA_site      2612..2625
                     /label=polya
     misc_feature    2585..2625
                     /label=BBa_B0012
     terminator      2585..2625
                     /label=BBa_B0012
     misc_feature    1690..2625
                     /label=BBa_I0462
     misc_feature    1482..2625
                     /label=BBa_K109702
     misc_feature    750..2625
                     /label=BBa_K199173
ORIGIN
        1 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
       61 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      121 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      181 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      241 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      301 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      361 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      421 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      481 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      541 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      601 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      661 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      721 gatgaactat acaaataata atactagaga aagaggagaa atactagatg gcttcctccg
      781 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
      841 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
      901 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
      961 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
     1021 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
     1081 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
     1141 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
     1201 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
     1261 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa
     1321 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
     1381 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
     1441 cttaataacg ctgatagtgc tagtgtagat cgctactaga gcaatacgca aaccgcctct
     1501 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     1561 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1621 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1681 atactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga
     1741 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat
     1801 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg
     1861 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat
     1921 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca
     1981 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa
     2041 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat
     2101 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt
     2161 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa
     2221 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta
     2281 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag
     2341 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc
     2401 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa
     2461 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg
     2521 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2581 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//