##gff-version 3
##sequence-region BBa_S04473 1 2625
BBa_S04473	.	engineered_region	1	741	.	+	0	ID=BBa_E5500;Name=BBa_E5500
BBa_S04473	.	CDS	22	741	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5500
BBa_S04473	.	CDS	22	741	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04473	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E5500
BBa_S04473	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04473	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04473	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04473	.	engineered_region	750	2625	.	+	0	ID=BBa_K199173;Name=BBa_K199173
BBa_S04473	.	engineered_region	1482	2625	.	+	0	ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199173
BBa_S04473	.	promoter	1482	1681	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_S04473	.	CDS	1482	1569	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04473	.	engineered_region	1482	1681	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04473	.	non_covalent_binding_site	1570	1607	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04473	.	start_codon	1654	1654	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04473	.	non_covalent_binding_site	1647	1681	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04473	.	promoter	1642	1647	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04473	.	promoter	1618	1623	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04473	.	engineered_region	1690	2625	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_S04473	.	terminator	2585	2625	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_S04473	.	engineered_region	2585	2625	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04473	.	polyA_site	2612	2625	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04473	.	stop_codon	2618	2618	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04473	.	stem_loop	2592	2611	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04473	.	CDS	1708	2463	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_S04473	.	point_mutation	2199	2199	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04473	.	engineered_tag	2464	2488	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04473	.	engineered_region	1708	2463	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04473	.	CDS	1708	2457	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04473	.	point_mutation	1881	1881	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04473	.	engineered_region	1708	1709	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04473	.	ribosome_entry_site	1690	1701	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_S04473	.	conserved_region	1694	1697	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04473	.	terminator	2497	2576	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_S04473	.	engineered_region	2497	2576	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04473	.	stem_loop	2508	2551	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04473	.	engineered_region	750	1473	.	+	0	ID=BBa_S04419;Name=BBa_S04419;Parent=BBa_K199173
BBa_S04473	.	ribosome_entry_site	750	761	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04419
BBa_S04473	.	conserved_region	754	757	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04473	.	CDS	768	1473	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_S04419
BBa_S04473	.	CDS	768	1442	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04473	.	engineered_tag	1449	1473	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_S04473
attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatg
gtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatt
tatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcg
agatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatat
ttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtt
aaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatac
atcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaac
tagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacaca
atctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattaca
catggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcaatacgcaaaccgcctctc
cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa
ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgt
gagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacataca
gaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtaca
ttgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccct
aaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcatt
caccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatc
aggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaa
aaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataatt
atcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatg
cgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcg
caaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatact
ttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata