LOCUS       BBa_S04474              2148 bp    DNA     linear       16-Dec-2024
DEFINITION  S04446:K315020
ACCESSION   BBa_S04474
VERSION     BBa_S04474.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     misc_feature    209..932
                     /label=BBa_I13502
     misc_feature    1..932
                     /label=BBa_I715039
     misc_feature    945..948
                     /label=conserved
     RBS             941..952
                     /label=BBa_B0034
     misc_feature    1..952
                     /label=BBa_S04446
     CDS             959..2148
                     /label=BBa_K315020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat
      961 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat
     1021 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat
     1081 cgccagtcac tatggcgtgc tgctagcctg tacgcgctga tgcagttcct gtgcgcgccg
     1141 gttctgggtg cgctgtctga ccgtttcggt cgtcgtccgg ttctgctggc gtctctgctg
     1201 ggtgcgacca tcgactacgc gatcatggcg accaccccgg ttctgtggat cctctacgcc
     1261 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     1321 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     1381 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     1441 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     1501 caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc
     1561 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     1621 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     1681 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     1741 gctcaagcct tcgtcactgg ccccgccacc aaacgtttcg gcgagaagca ggccattatc
     1801 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     1861 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     1921 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     1981 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     2041 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     2101 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctaa
//