LOCUS BBa_S04481 4663 bp DNA linear 16-Dec-2024 DEFINITION S04470:J31007 ACCESSION BBa_S04481 VERSION BBa_S04481.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 1246..1284 /label=LVA CDS 157..1284 /label=lacI-LVA misc_feature 157..1284 /label=BBa_C0012 misc_feature 1285..1309 /label=Help:Barcodes CDS 157..1284 /label=BBa_C0012 misc_feature 139..1309 /label=BBa_S0100 promoter 1..1309 /label=BBa_K091225 CDS 1318..1405 /label=end of LacI coding region (inactive) misc_feature 1318..1517 /label=BBa_R0010 misc_feature 1406..1443 /label=CAP binding site promoter 1454..1459 /label=-35 promoter 1478..1483 /label=-10 misc_feature 1483..1517 /label=LacI binding site misc_feature 1490..1490 /label=start promoter 1318..1517 /label=BBa_R0010 misc_feature 1530..1533 /label=conserved RBS 1526..1537 /label=BBa_B0034 CDS 1544..2218 /label=mrfp1 misc_feature 2225..2249 /label=Help:Barcodes CDS 1544..2224 /label=BBa_E1010 misc_feature 1526..2249 /label=BBa_I13502 misc_feature 1318..2249 /label=BBa_I715039 misc_feature 2262..2265 /label=conserved RBS 2258..2269 /label=BBa_B0034 misc_feature 1318..2269 /label=BBa_S04446 CDS 2276..3466 /label=tetA(C) forward primer_bind 2276..2299 /label=Fwd primer J31007 primer_bind 3447..3466 /label=Rev primer J31007 misc_feature 3464..3466 /label=stop CDS 2276..3466 /label=BBa_J31007 misc_feature 1318..3466 /label=BBa_S04448 misc_feature 1..3466 /label=BBa_S04470 CDS 3473..4663 /label=tetA(C) forward primer_bind 3473..3496 /label=Fwd primer J31007 primer_bind 4644..4663 /label=Rev primer J31007 misc_feature 4661..4663 /label=stop CDS 3473..4663 /label=BBa_J31007 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 181 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 241 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 301 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 361 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 421 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 481 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 541 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 601 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 661 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 721 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 781 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 841 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 901 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 961 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 1021 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 1081 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 1141 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 1201 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 1261 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcaa 1321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 1381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 1441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 1501 gataacaatt tcacacatac tagagaaaga ggagaaatac tagatggctt cctccgaaga 1561 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga 1621 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact 1681 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca 1741 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc 1801 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac 1861 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg 1921 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc 1981 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa 2041 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc 2101 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga 2161 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta 2221 ataacgctga tagtgctagt gtagatcgct actagagaaa gaggagaaat actagatgaa 2281 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg 2341 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc 2401 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt 2461 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg 2521 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg 2581 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 2641 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 2701 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc 2761 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 2821 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc 2881 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 2941 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg 3001 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc 3061 tcaagccttc gtcactggcc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc 3121 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat 3181 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc 3241 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc 3301 tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc 3361 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct 3421 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctaatact agatgaaatc 3481 taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt 3541 ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag 3601 tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct 3661 cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc 3721 cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg 3781 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat 3841 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg 3901 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt 3961 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga 4021 gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt 4081 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca 4141 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg 4201 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca 4261 agccttcgtc actggccccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg 4321 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc 4381 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat 4441 gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct 4501 taccagccta acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc 4561 gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc 4621 cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc taa //