LOCUS BBa_S04482 4519 bp DNA linear 16-Dec-2024 DEFINITION S04471:J31007 ACCESSION BBa_S04482 VERSION BBa_S04482.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 1246..1284 /label=LVA CDS 157..1284 /label=lacI-LVA misc_feature 157..1284 /label=BBa_C0012 misc_feature 1285..1309 /label=Help:Barcodes CDS 157..1284 /label=BBa_C0012 misc_feature 139..1309 /label=BBa_S0100 promoter 1..1309 /label=BBa_K091225 promoter 1334..1339 /label=-35 promoter 1361..1366 /label=-10 promoter 1318..1373 /label=BBa_K091110 misc_feature 1386..1389 /label=conserved RBS 1382..1393 /label=BBa_B0034 CDS 1400..2074 /label=mrfp1 misc_feature 2081..2105 /label=Help:Barcodes CDS 1400..2105 /label=BBa_E1010 misc_feature 1382..2105 /label=BBa_J70390 misc_feature 1318..2105 /label=BBa_K315008 misc_feature 2118..2121 /label=conserved RBS 2114..2125 /label=BBa_B0034 CDS 2132..3322 /label=tetA(C) forward primer_bind 2132..2155 /label=Fwd primer J31007 primer_bind 3303..3322 /label=Rev primer J31007 misc_feature 3320..3322 /label=stop CDS 2132..3322 /label=BBa_J31007 misc_feature 2114..3322 /label=BBa_J85100 misc_feature 1318..3322 /label=BBa_K315009 misc_feature 1..3322 /label=BBa_S04471 CDS 3329..4519 /label=tetA(C) forward primer_bind 3329..3352 /label=Fwd primer J31007 primer_bind 4500..4519 /label=Rev primer J31007 misc_feature 4517..4519 /label=stop CDS 3329..4519 /label=BBa_J31007 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 181 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 241 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 301 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 361 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 421 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 481 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 541 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 601 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 661 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 721 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 781 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 841 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 901 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 961 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 1021 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 1081 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 1141 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 1201 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 1261 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcgt 1321 tgacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggtactaga 1381 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt 1441 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga 1501 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct 1561 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa 1621 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga 1681 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca 1741 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc 1801 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga 1861 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga 1921 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa 1981 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta 2041 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag 2101 atcgctacta gagaaagagg agaaatacta gatgaaatct aacaatgcgc tcatcgtcat 2161 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg 2221 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc 2281 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt 2341 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat 2401 ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg 2461 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc 2521 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 2581 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa 2641 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg 2701 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 2761 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 2821 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 2881 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggccccgc 2941 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg 3001 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct 3061 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga 3121 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat 3181 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 3241 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 3301 gagccgggcc acctcgacct aatactagat gaaatctaac aatgcgctca tcgtcatcct 3361 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct 3421 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct 3481 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg 3541 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc 3601 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc 3661 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg 3721 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg 3781 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg 3841 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc 3901 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 3961 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 4021 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc 4081 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gccccgccac 4141 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta 4201 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc 4261 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga 4321 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg 4381 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg 4441 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag 4501 ccgggccacc tcgacctaa //