LOCUS BBa_S04487 828 bp DNA linear 05-Nov-2024 DEFINITION S04460:K093005 ACCESSION BBa_S04487 VERSION BBa_S04487.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 63..96 /label=BBa_K315004 misc_feature 1..96 /label=BBa_S04460 misc_feature 109..112 /label=conserved RBS 105..116 /label=BBa_B0034 CDS 123..797 /label=mrfp1 misc_feature 804..828 /label=Help:Barcodes CDS 123..828 /label=BBa_E1010 misc_feature 105..828 /label=BBa_K093005 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agataacttc gtataagaaa ccatatacga agttattact agagaaagag gagaaatact 121 agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag 181 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag 241 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca 301 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc 361 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg 421 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct 481 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa 541 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg 601 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca 661 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg 721 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc 781 gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgc //