LOCUS       BBa_S04488              2100 bp    DNA     linear       23-Dec-2024
DEFINITION  J23107:I746101
ACCESSION   BBa_S04488
VERSION     BBa_S04488.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23107
     RBS             44..55
                     /label=B0034-sequence RBS
     RBS             1316..1327
                     /label=B0034-sequence RBS
     misc_feature    1310..1315
                     /label=BamHI site
     CDS             62..1309
                     /label=agrC coding sequence
     CDS             1334..1963
                     /label=agrA coding sequence
     misc_feature    44..1963
                     /label=BBa_I746100
     stem_loop       1983..2026
                     /label=stem_loop
     misc_feature    1972..2051
                     /label=BBa_B0010
     terminator      1972..2051
                     /label=BBa_B0010
     stem_loop       2067..2086
                     /label=T7 TE
     misc_feature    2093..2093
                     /label=stop
     polyA_site      2087..2100
                     /label=polya
     misc_feature    2060..2100
                     /label=BBa_B0012
     terminator      2060..2100
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgattctg atgttcacca ttccagcaat tattagcggt attaagtaca gcaagctgga
      121 ttatttcttc atcatcgtaa tctctaccct gtccctgttc ctgttcaaaa tgttcgattc
      181 cgcgagcctg attatcctga cttccttcat catcatcatg tatttcgtaa aaatcaaatg
      241 gtatagcatc ctgctgatca tgacctccca gattatcctg tactgtgcca actacatgta
      301 tatcgttatc tacgcgtaca tcacgaaaat ctccgattct atcttcgtta tctttccgtc
      361 tttcttcgtg gtgtacgtta cgatctctat tctgttttcc tatattatca atcgcgttct
      421 gaaaaagatc tctactccgt acctgattct gaacaaaggt tttctgatcg tcatctccac
      481 tatcctgctg ctgaccttct ctctgttctt cttttactct cagatcaact ccgatgaggc
      541 aaaagtcatc cgtcagtaca gcttcatctt tatcggtatt actatcttcc tgagcattct
      601 gactttcgtt atcagccagt tcctgctgaa agaaatgaag tacaagcgca accaagaaga
      661 aatcgagact tactacgagt acaccctgaa gattgaagcg atcaacaacg aaatgcgtaa
      721 atttcgtcac gactacgtta acattctgac caccctgagc gaatacatcc gtgaagacga
      781 catgcctggc ctgcgtgatt atttcaacaa aaacattgtg ccgatgaaag ataacctgca
      841 aatgaacgct attaagctga acggtatcga aaacctgaaa gtacgtgaga tcaagggtct
      901 gattaccgct aaaatcctgc gtgcgcagga aatgaacatc ccaattagca tcgaaatccc
      961 agacgaagtt tctagcatta acctgaacat gattgacctg tctcgtagca tcggcatcat
     1021 cctggacaac gctatcgaag cttccactga aatcgacgac ccgattattc gcgttgcttt
     1081 tatcgagagc gaaaactccg tgacctttat cgtgatgaac aaatgcgcgg acgatatccc
     1141 gcgtattcac gaactgttcc aggaatcttt cagcaccaag ggtgaaggtc gcggcctggg
     1201 cctgtccacc ctgaaagaga tcgcggataa cgccgacaac gttctgctgg acactattat
     1261 cgaaaacggt ttcttcattc agaaagtcga aatcatcaat aactaataag gatccaaaga
     1321 ggagaaatac tagatggaaa tcgcactggc tacggacaat ccatatgagg ttctggaaca
     1381 ggctaaaaac atgaacgaca tcggctgcta tttcctggat attcagctga gcaccgacat
     1441 caacggtatc aaactgggct ctgaaatccg taaacatgat ccggttggta acattatttt
     1501 cgtgacttct cattctgagc tgacctatct gacgttcgta tacaaggttg cagccatgga
     1561 tttcatcttt aaagacgatc cggcagagct gcgtacgcgc atcatcgact gtctggaaac
     1621 tgcgcacacg cgcctgcaac tgctgtccaa agataactcc gtggaaacca ttgaactgaa
     1681 acgtggcagc aacagcgttt acgttcagta cgatgatatc atgttcttcg aaagctccac
     1741 taagtcccac cgtctgatcg cgcatctgga taatcgtcag atcgagttct atggcaacct
     1801 gaaagagctg agccagctgg acgatcgctt cttccgttgt cataactcct tcgttgttaa
     1861 ccgtcacaac atcgaatcca tcgactccaa agagcgtatt gtatacttca agaacaaaga
     1921 acactgttat gcgtctgtac gcaatgtcaa gaaaatctaa taatactaga gccaggcatc
     1981 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2041 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//