##gff-version 3
##sequence-region BBa_S04493 1 870
BBa_S04493	.	engineered_region	1	828	.	+	0	ID=BBa_S04487;Name=BBa_S04487
BBa_S04493	.	engineered_region	1	96	.	+	0	ID=BBa_S04460;Name=BBa_S04460;Parent=BBa_S04487
BBa_S04493	.	sequence_feature	63	96	.	+	0	ID=BBa_K315004;Name=BBa_K315004;Parent=BBa_S04460
BBa_S04493	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S04460
BBa_S04493	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04493	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04493	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04493	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04493	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04493	.	engineered_region	105	828	.	+	0	ID=BBa_K093005;Name=BBa_K093005;Parent=BBa_S04487
BBa_S04493	.	CDS	123	828	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K093005
BBa_S04493	.	CDS	123	797	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04493	.	engineered_tag	804	828	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04493	.	ribosome_entry_site	105	116	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K093005
BBa_S04493	.	conserved_region	109	112	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04493	.	sequence_feature	837	870	.	+	0	ID=BBa_K315012;Name=BBa_K315012
BBa_S04493	.	sequence_feature	850	857	.	+	0	ID=annotation2071523;Name=spacer region;Parent=BBa_K315012
BBa_S04493	.	sequence_feature	858	870	.	+	0	ID=annotation2071524;Name=inverted repeat arm;Parent=BBa_K315012
BBa_S04493	.	sequence_feature	837	849	.	+	0	ID=annotation2071522;Name=inverted repeat arm;Parent=BBa_K315012
BBa_S04493	.	sequence_alteration	851	851	.	+	0	ID=annotation2071535;Name=T -> A;Parent=BBa_K315012
BBa_S04493	.	sequence_alteration	852	852	.	+	0	ID=annotation2071536;Name=G -> C;Parent=BBa_K315012
BBa_S04493	.	sequence_alteration	856	856	.	+	0	ID=annotation2071537;Name=G -> A;Parent=BBa_K315012
>BBa_S04493
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataagaa
accatatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatg
cgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacg
aaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagtt
ccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttc
aaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagt
tcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggga
agcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggt
ggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgaca
tcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccac
cggtgcttaataacgctgatagtgctagtgtagatcgctactagagataacttcgtataaactatactatacgaagttat
t