BBa_S04493
type: ComponentDefinition
persistent identity: BBa S04493
display id: BBa_S04493
version: 1
was generated by: iGEM to SBOL conversion
description: S04487:K315012
created: 2010-07-29T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7379
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Shuman
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S04487
component: BBa_K315012
sequence annotation: BBa_S04487
sequence annotation: BBa_K315012
sequence: https://synbiohub.org/public/igem/BBa_S04493_sequence/1

BBa_S04487
type: Component
persistent identity: component2257739
display id: component2257739
version: 1
top level: BBa_S04493
definition: BBa_S04487
access: public

BBa_K315012
type: Component
persistent identity: component2257746
display id: component2257746
version: 1
top level: BBa_S04493
definition: BBa_K315012
access: public

BBa_S04487
type: SequenceAnnotation
persistent identity: annotation2257739
display id: annotation2257739
version: 1
top level: BBa_S04493
location: https://synbiohub.org/public/igem/BBa_S04493/annotation2257739/range2257739/1
component: BBa_S04487

https://synbiohub.org/public/igem/BBa_S04493/annotation2257739/range2257739/1
type: Range
persistent identity: range2257739
display id: range2257739
version: 1
top level: BBa_S04493
direction: direction/reverse
start: 1
end: 828
orientation: inline

BBa_K315012
type: SequenceAnnotation
persistent identity: annotation2257746
display id: annotation2257746
version: 1
top level: BBa_S04493
location: https://synbiohub.org/public/igem/BBa_S04493/annotation2257746/range2257746/1
component: BBa_K315012

https://synbiohub.org/public/igem/BBa_S04493/annotation2257746/range2257746/1
type: Range
persistent identity: range2257746
display id: range2257746
version: 1
top level: BBa_S04493
direction: direction/reverse
start: 837
end: 870
orientation: inline

BBa_K315012
type: ComponentDefinition
persistent identity: BBa K315012
display id: BBa_K315012
version: 1
was generated by: iGEM to SBOL conversion
description: LoxBri (F) Variant forward lox site with 3 base changes in the spacer region
created: 2010-06-23T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: We learned about the use of variant lox sites to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html). We discovered this specific sequence in "Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination" (http://www.ncbi.nlm.nih.gov/pubmed/9714735). [edit]
owned by: james
owned by: myers
mutable description: The cre-lox system is used for site-specific recombination. Lox sites are 34 bp sequences of DNA that flank coding sequences. When cre recombinase is introduced, it can excise or invert the portions of DNA between two lox sites. If the two lox sites flanking the coding sequence are both forward or both reverse, the coding sequence will be excised, leaving one lox site behind. If one site is forward and the other reverse, the coding sequence will be inverted.
discontinued: false
dominant: false
group u list: _435_
m user id: 0
owner id: 7370
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.
star: false
creator: Brianna Pearson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: inverted repeat arm
sequence annotation: spacer region
sequence annotation: T -> A
sequence annotation: G -> C
sequence annotation: G -> A
sequence: https://synbiohub.org/public/igem/BBa_K315012_sequence/1

inverted repeat arm
type: SequenceAnnotation
persistent identity: annotation2071522
display id: annotation2071522
version: 1
top level: BBa_K315012
location: https://synbiohub.org/public/igem/BBa_K315012/annotation2071522/range2071522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K315012/annotation2071522/range2071522/1
type: Range
persistent identity: range2071522
display id: range2071522
version: 1
top level: BBa_K315012
direction: direction/forward
start: 1
end: 13
orientation: inline

spacer region
type: SequenceAnnotation
persistent identity: annotation2071523
display id: annotation2071523
version: 1
top level: BBa_K315012
location: https://synbiohub.org/public/igem/BBa_K315012/annotation2071523/range2071523/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K315012/annotation2071523/range2071523/1
type: Range
persistent identity: range2071523
display id: range2071523
version: 1
top level: BBa_K315012
direction: direction/forward
start: 14
end: 21
orientation: inline

T -> A
type: SequenceAnnotation
persistent identity: annotation2071535
display id: annotation2071535
version: 1
top level: BBa_K315012
location: https://synbiohub.org/public/igem/BBa_K315012/annotation2071535/range2071535/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K315012/annotation2071535/range2071535/1
type: Range
persistent identity: range2071535
display id: range2071535
version: 1
top level: BBa_K315012
direction: direction/forward
start: 15
end: 15
orientation: inline

G -> C
type: SequenceAnnotation
persistent identity: annotation2071536
display id: annotation2071536
version: 1
top level: BBa_K315012
location: https://synbiohub.org/public/igem/BBa_K315012/annotation2071536/range2071536/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K315012/annotation2071536/range2071536/1
type: Range
persistent identity: range2071536
display id: range2071536
version: 1
top level: BBa_K315012
direction: direction/forward
start: 16
end: 16
orientation: inline

G -> A
type: SequenceAnnotation
persistent identity: annotation2071537
display id: annotation2071537
version: 1
top level: BBa_K315012
location: https://synbiohub.org/public/igem/BBa_K315012/annotation2071537/range2071537/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K315012/annotation2071537/range2071537/1
type: Range
persistent identity: range2071537
display id: range2071537
version: 1
top level: BBa_K315012
direction: direction/forward
start: 20
end: 20
orientation: inline

inverted repeat arm
type: SequenceAnnotation
persistent identity: annotation2071524
display id: annotation2071524
version: 1
top level: BBa_K315012
location: https://synbiohub.org/public/igem/BBa_K315012/annotation2071524/range2071524/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K315012/annotation2071524/range2071524/1
type: Range
persistent identity: range2071524
display id: range2071524
version: 1
top level: BBa_K315012
direction: direction/forward
start: 22
end: 34
orientation: inline

BBa_S04487
type: ComponentDefinition
persistent identity: BBa S04487
display id: BBa_S04487
version: 1
was generated by: iGEM to SBOL conversion
description: S04460:K093005
created: 2010-07-23T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7379
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Shuman
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S04460
component: BBa_K093005
sequence annotation: BBa_S04460
sequence annotation: BBa_K093005
sequence: https://synbiohub.org/public/igem/BBa_S04487_sequence/1

BBa_S04460
type: Component
persistent identity: component2250255
display id: component2250255
version: 1
top level: BBa_S04487
definition: BBa_S04460
access: public

BBa_K093005
type: Component
persistent identity: component2250261
display id: component2250261
version: 1
top level: BBa_S04487
definition: RBS-RFP
access: public

BBa_S04460
type: SequenceAnnotation
persistent identity: annotation2250255
display id: annotation2250255
version: 1
top level: BBa_S04487
location: https://synbiohub.org/public/igem/BBa_S04487/annotation2250255/range2250255/1
component: BBa_S04460

https://synbiohub.org/public/igem/BBa_S04487/annotation2250255/range2250255/1
type: Range
persistent identity: range2250255
display id: range2250255
version: 1
top level: BBa_S04487
direction: direction/reverse
start: 1
end: 96
orientation: inline

BBa_K093005
type: SequenceAnnotation
persistent identity: annotation2250261
display id: annotation2250261
version: 1
top level: BBa_S04487
location: https://synbiohub.org/public/igem/BBa_S04487/annotation2250261/range2250261/1
component: BBa_K093005

https://synbiohub.org/public/igem/BBa_S04487/annotation2250261/range2250261/1
type: Range
persistent identity: range2250261
display id: range2250261
version: 1
top level: BBa_S04487
direction: direction/reverse
start: 105
end: 828
orientation: inline

RBS-RFP
type: ComponentDefinition
persistent identity: BBa K093005
display id: BBa_K093005
version: 1
was generated by: iGEM to SBOL conversion
description: RFP with RBS
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: registry plasmids
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RFP, BBa_E1010, with Elowitz RBS, BBa_B0034.
discontinued: false
dominant: false
experience: experience/Works
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The part was needed for downstream applications. There can be no expression of a reporter without a ribosome binding site.
star: true
creator: Kathy Lam, Danielle Nash
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K093005_sequence/1

BBa_B0034
type: Component
persistent identity: component2244022
display id: component2244022
version: 1
top level: RBS-RFP
definition: BBa_B0034
access: public

BBa_E1010
type: Component
persistent identity: component2244025
display id: component2244025
version: 1
top level: RBS-RFP
definition: mRFP1
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244022
display id: annotation2244022
version: 1
top level: RBS-RFP
location: https://synbiohub.org/public/igem/BBa_K093005/annotation2244022/range2244022/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K093005/annotation2244022/range2244022/1
type: Range
persistent identity: range2244022
display id: range2244022
version: 1
top level: RBS-RFP
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2244025
display id: annotation2244025
version: 1
top level: RBS-RFP
location: https://synbiohub.org/public/igem/BBa_K093005/annotation2244025/range2244025/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K093005/annotation2244025/range2244025/1
type: Range
persistent identity: range2244025
display id: range2244025
version: 1
top level: RBS-RFP
direction: direction/reverse
start: 19
end: 724
orientation: inline

BBa_S04460
type: ComponentDefinition
persistent identity: BBa S04460
display id: BBa_S04460
version: 1
was generated by: iGEM to SBOL conversion
description: R0040:K315004
created: 2010-07-12T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7379
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Shuman
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_R0040
component: BBa_K315004
sequence annotation: BBa_R0040
sequence annotation: BBa_K315004
sequence: https://synbiohub.org/public/igem/BBa_S04460_sequence/1

BBa_R0040
type: Component
persistent identity: component2073306
display id: component2073306
version: 1
top level: BBa_S04460
definition: p(tetR)
access: public

BBa_K315004
type: Component
persistent identity: component2073311
display id: component2073311
version: 1
top level: BBa_S04460
definition: BBa_K315004
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2073306
display id: annotation2073306
version: 1
top level: BBa_S04460
location: https://synbiohub.org/public/igem/BBa_S04460/annotation2073306/range2073306/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S04460/annotation2073306/range2073306/1
type: Range
persistent identity: range2073306
display id: range2073306
version: 1
top level: BBa_S04460
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K315004
type: SequenceAnnotation
persistent identity: annotation2073311
display id: annotation2073311
version: 1
top level: BBa_S04460
location: https://synbiohub.org/public/igem/BBa_S04460/annotation2073311/range2073311/1
component: BBa_K315004

https://synbiohub.org/public/igem/BBa_S04460/annotation2073311/range2073311/1
type: Range
persistent identity: range2073311
display id: range2073311
version: 1
top level: BBa_S04460
direction: direction/reverse
start: 63
end: 96
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K315004
type: ComponentDefinition
persistent identity: BBa K315004
display id: BBa_K315004
version: 1
was generated by: iGEM to SBOL conversion
description: variant forward lox site with 3 base changes in the spacer region (loxm2 forward)
created: 2010-06-15T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: We learned about the use of variant lox sites to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html). We discovered this specific sequence in "Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination" (http://www.ncbi.nlm.nih.gov/pubmed/9714735).
owned by: james
owned by: myers
mutable description: The cre-lox system is used for site-specific recombination. Lox sites are 34 bp sequences of DNA that flank coding sequences. When cre recombinase is introduced, it can excise or invert the portions of DNA between two lox sites. If the two lox sites flanking the coding sequence are both forward or both reverse, the coding sequence will be excised, leaving one lox site behind. If the one site is forward and the other reverse, the coding sequence will be inverted.
discontinued: false
dominant: false
group u list: _435_
m user id: 0
owner id: 7379
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.
star: false
creator: Tom Shuman
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K315004_sequence/1

https://synbiohub.org/public/igem/BBa_S04493_sequence/1
type: Sequence
persistent identity: BBa S04493 sequence
display id: BBa_S04493_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataagaaaccatatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagataacttcgtataaactatactatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K315012_sequence/1
type: Sequence
persistent identity: BBa K315012 sequence
display id: BBa_K315012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataaactatactatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04487_sequence/1
type: Sequence
persistent identity: BBa S04487 sequence
display id: BBa_S04487_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataagaaaccatatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093005_sequence/1
type: Sequence
persistent identity: BBa K093005 sequence
display id: BBa_K093005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04460_sequence/1
type: Sequence
persistent identity: BBa S04460 sequence
display id: BBa_S04460_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataagaaaccatatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K315004_sequence/1
type: Sequence
persistent identity: BBa K315004 sequence
display id: BBa_K315004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataagaaaccatatacgaagttat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z