LOCUS       BBa_S04496              1419 bp    DNA     linear       05-Nov-2024
DEFINITION  S03511:K315020
ACCESSION   BBa_S04496
VERSION     BBa_S04496.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..1419
                     /label=BBa_K315020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgaaatctaa
      241 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
      301 tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca
      361 ctatggcgtg ctgctagcct gtacgcgctg atgcagttcc tgtgcgcgcc ggttctgggt
      421 gcgctgtctg accgtttcgg tcgtcgtccg gttctgctgg cgtctctgct gggtgcgacc
      481 atcgactacg cgatcatggc gaccaccccg gttctgtgga tcctctacgc cggacgcatc
      541 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
      601 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
      661 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
      721 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
      781 cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
      841 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
      901 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
      961 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     1021 ttcgtcactg gccccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     1081 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     1141 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     1201 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     1261 agcctaactt cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc
     1321 acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg
     1381 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctaa
//