LOCUS       BBa_S04497              1420 bp    DNA     linear       22-Nov-2024
DEFINITION  S03511:K315021
ACCESSION   BBa_S04497
VERSION     BBa_S04497.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..1420
                     /label=BBa_K315021
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgaaatctaa
      241 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
      301 tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca
      361 ctatggcgtg ctgctagcgc tatatgccct aatgcaattt ctatgtgccc ccgtcctagg
      421 agccctaagt gataggtttg gaaggaggcc cgtcctacta gccagtctac taggagccac
      481 aatagattat gccataatgg ccacaacacc cgtcctatgg atcctctacg ccggacgcat
      541 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
      601 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
      661 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
      721 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
      781 gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
      841 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
      901 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
      961 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     1021 cttcgtcact ggccccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     1081 ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     1141 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     1201 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac
     1261 cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag
     1321 cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc
     1381 gttgcgtcgc ggtgcatgga gccgggccac ctcgacctaa
//