LOCUS       BBa_S04498              1540 bp    DNA     linear       15-Dec-2024
DEFINITION  K091225:S03511
ACCESSION   BBa_S04498
VERSION     BBa_S04498.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     promoter        232..361
                     /label=BBa_I13453
     misc_feature    374..377
                     /label=conserved
     RBS             370..381
                     /label=BBa_B0034
     misc_feature    1477..1515
                     /label=LVA
     CDS             388..1515
                     /label=lacI-LVA
     misc_feature    388..1515
                     /label=BBa_C0012
     misc_feature    1516..1540
                     /label=Help:Barcodes
     CDS             388..1515
                     /label=BBa_C0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga gacattgatt
      241 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
      301 ctacctgacg ctttttatcg caactctcta ctgtttctcc ataccgtttt tttgggctag
      361 ctactagaga aagaggagaa atactagatg gtgaatgtga aaccagtaac gttatacgat
      421 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
      481 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
      541 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
      601 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
      661 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
      721 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
      781 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
      841 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
      901 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
      961 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
     1021 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
     1081 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     1141 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
     1201 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     1261 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     1321 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     1381 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1441 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct
     1501 ttagtagctt aataactctg atagtgctag tgtagatctc
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