LOCUS       BBa_S04499              1540 bp    DNA     linear       15-Dec-2024
DEFINITION  K091225:S03511
ACCESSION   BBa_S04499
VERSION     BBa_S04499.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    1246..1284
                     /label=LVA
     CDS             157..1284
                     /label=lacI-LVA
     misc_feature    157..1284
                     /label=BBa_C0012
     misc_feature    1285..1309
                     /label=Help:Barcodes
     CDS             157..1284
                     /label=BBa_C0012
     misc_feature    139..1309
                     /label=BBa_S0100
     promoter        1..1309
                     /label=BBa_K091225
     CDS             1318..1405
                     /label=end of LacI coding region (inactive)
     misc_feature    1318..1517
                     /label=BBa_R0010
     misc_feature    1406..1443
                     /label=CAP binding site
     promoter        1454..1459
                     /label=-35
     promoter        1478..1483
                     /label=-10
     misc_feature    1483..1517
                     /label=LacI binding site
     misc_feature    1490..1490
                     /label=start
     promoter        1318..1517
                     /label=BBa_R0010
     RBS             1526..1540
                     /label=RBS-1\Strong
     RBS             1533..1537
                     /label=RBS
     misc_feature    1526..1540
                     /label=BBa_B0030
     RBS             1526..1540
                     /label=BBa_B0030
     misc_feature    1318..1540
                     /label=BBa_S03511
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg
      181 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
      241 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
      301 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
      361 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
      421 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
      481 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
      541 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
      601 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
      661 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
      721 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
      781 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
      841 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
      901 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
      961 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
     1021 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     1081 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
     1141 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1201 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa
     1261 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcaa
     1321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     1441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     1501 gataacaatt tcacacatac tagagattaa agaggagaaa
//