##gff-version 3
##sequence-region BBa_S04499 1 1540
BBa_S04499	.	promoter	1	1309	.	+	0	ID=BBa_K091225;Name=BBa_K091225
BBa_S04499	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091225
BBa_S04499	.	engineered_region	139	1309	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_K091225
BBa_S04499	.	CDS	157	1284	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_S04499	.	engineered_tag	1285	1309	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04499	.	CDS	157	1284	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S04499	.	tag	1246	1284	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04499	.	engineered_region	157	1284	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04499	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_S04499	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04499	.	engineered_region	1318	1540	.	+	0	ID=BBa_S03511;Name=BBa_S03511
BBa_S04499	.	promoter	1318	1517	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_S03511
BBa_S04499	.	non_covalent_binding_site	1483	1517	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04499	.	non_covalent_binding_site	1406	1443	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04499	.	engineered_region	1318	1517	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04499	.	promoter	1454	1459	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04499	.	CDS	1318	1405	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04499	.	start_codon	1490	1490	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04499	.	promoter	1478	1483	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04499	.	ribosome_entry_site	1526	1540	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_S03511
BBa_S04499	.	engineered_region	1526	1540	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04499	.	ribosome_entry_site	1533	1537	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04499	.	ribosome_entry_site	1526	1540	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_S04499
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtg
aaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaacc
gcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtc
gcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggc
gtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatg
accaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccat
caacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatc
gcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatc
aaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatga
gggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccggg
ctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaacca
ccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaa
gggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgc
gcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaact
acgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcaatacgcaaaccgcctctccccgcg
cgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatg
tgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgg
ataacaatttcacacatactagagattaaagaggagaaa