LOCUS BBa_S04502 1416 bp DNA linear 25-Nov-2024 DEFINITION J23104:Q01121 ACCESSION BBa_S04502 VERSION BBa_S04502.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 misc_feature 50..53 /label=conserved RBS 44..57 /label=BBa_B0031 misc_feature 1153..1191 /label=LVA CDS 64..1191 /label=lacI-LVA misc_feature 64..1191 /label=BBa_C0012 misc_feature 1192..1216 /label=Help:Barcodes CDS 64..1191 /label=BBa_C0012 stem_loop 1236..1279 /label=stem_loop misc_feature 1225..1304 /label=BBa_B0010 terminator 1225..1304 /label=BBa_B0010 stem_loop 1320..1339 /label=T7 TE misc_feature 1346..1346 /label=stop polyA_site 1340..1353 /label=polya misc_feature 1313..1353 /label=BBa_B0012 terminator 1313..1353 /label=BBa_B0012 terminator 1225..1353 /label=BBa_B0015 misc_feature 1364..1380 /label=lac O1 promoter 1381..1386 /label=-35 misc_feature 1387..1403 /label=lac O1 promoter 1404..1409 /label=-10 misc_feature 1362..1415 /label=BBa_R0011 promoter 1362..1415 /label=BBa_R0011 misc_feature 44..1416 /label=BBa_Q01121 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaacctac 61 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct 121 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa 181 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg 241 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg 301 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 361 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg 421 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 481 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 541 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 601 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 661 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 721 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 781 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 841 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 901 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg 961 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 1021 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc 1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1141 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 1201 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 1261 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 1321 gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag cggataacaa 1381 ttgacattgt gagcggataa caagatactg agcaca // LOCUS BBa_S04500 1416 bp DNA linear 25-Nov-2024 DEFINITION J23104:Q01121 ACCESSION BBa_S04500 VERSION BBa_S04500.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 misc_feature 50..53 /label=conserved RBS 44..57 /label=BBa_B0031 misc_feature 1153..1191 /label=LVA CDS 64..1191 /label=lacI-LVA misc_feature 64..1191 /label=BBa_C0012 misc_feature 1192..1216 /label=Help:Barcodes CDS 64..1191 /label=BBa_C0012 stem_loop 1236..1279 /label=stem_loop misc_feature 1225..1304 /label=BBa_B0010 terminator 1225..1304 /label=BBa_B0010 stem_loop 1320..1339 /label=T7 TE misc_feature 1346..1346 /label=stop polyA_site 1340..1353 /label=polya misc_feature 1313..1353 /label=BBa_B0012 terminator 1313..1353 /label=BBa_B0012 misc_feature 1364..1380 /label=lac O1 promoter 1381..1386 /label=-35 misc_feature 1387..1403 /label=lac O1 promoter 1404..1409 /label=-10 misc_feature 1362..1415 /label=BBa_R0011 promoter 1362..1415 /label=BBa_R0011 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaacctac 61 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct 121 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa 181 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg 241 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg 301 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 361 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg 421 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 481 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 541 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 601 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 661 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 721 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 781 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 841 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 901 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg 961 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 1021 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc 1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1141 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 1201 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 1261 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 1321 gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag cggataacaa 1381 ttgacattgt gagcggataa caagatactg agcaca //