##gff-version 3
##sequence-region BBa_S04504 1 1883
BBa_S04504	.	engineered_region	1220	1883	.	+	0	ID=BBa_K081008;Name=BBa_K081008
BBa_S04504	.	ribosome_entry_site	1220	1234	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081008
BBa_S04504	.	ribosome_entry_site	1227	1231	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04504	.	engineered_region	1220	1234	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04504	.	ribosome_entry_site	1220	1234	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04504	.	CDS	1241	1858	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K081008
BBa_S04504	.	CDS	1241	1819	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04504	.	engineered_tag	1859	1883	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04504	.	engineered_region	1241	1858	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04504	.	tag	1820	1851	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04504	.	engineered_region	1	1211	.	+	0	ID=BBa_K329004;Name=BBa_K329004
BBa_S04504	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K329004
BBa_S04504	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_S04504	.	CDS	21	1211	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_K329004
BBa_S04504	.	primer_binding_site	1192	1211	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S04504	.	primer_binding_site	21	44	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S04504	.	CDS	21	1211	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S04504	.	stop_codon	1209	1211	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
>BBa_S04504
tcacacaggaaacctactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtagg
cataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggc
gtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgcc
gcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcct
ctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgat
ggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggg
gactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctg
cttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccgg
tgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcag
cgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaat
cttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggc
atggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttc
tcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagct
tcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcg
gcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtg
catggagccgggccacctcgacctaatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcg
gattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagt
gggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatga
tactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattg
cttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaaga
taaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacaga
atatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaa
gaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaa
atgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc