##gff-version 3
##sequence-region BBa_S04505 1 1881
BBa_S04505	.	engineered_region	1	1209	.	+	0	ID=BBa_K329003;Name=BBa_K329003
BBa_S04505	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K329003
BBa_S04505	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04505	.	CDS	19	1209	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_K329003
BBa_S04505	.	primer_binding_site	19	42	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S04505	.	primer_binding_site	1190	1209	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S04505	.	CDS	19	1209	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S04505	.	stop_codon	1207	1209	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S04505	.	engineered_region	1218	1881	.	+	0	ID=BBa_K081008;Name=BBa_K081008
BBa_S04505	.	ribosome_entry_site	1218	1232	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081008
BBa_S04505	.	engineered_region	1218	1232	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04505	.	ribosome_entry_site	1225	1229	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04505	.	ribosome_entry_site	1218	1232	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04505	.	CDS	1239	1856	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K081008
BBa_S04505	.	engineered_tag	1857	1881	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04505	.	tag	1818	1849	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04505	.	engineered_region	1239	1856	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04505	.	CDS	1239	1817	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
>BBa_S04505
aaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgt
gctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgc
ccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctct
acgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgct
tcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctc
gcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttc
aaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcgga
ttttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgg
gacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgata
ctgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgct
tggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagata
aataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaat
atgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaaga
aattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaat
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc