LOCUS       BBa_S04508               937 bp    DNA     linear       22-Dec-2024
DEFINITION  J13002:K300005
ACCESSION   BBa_S04508
VERSION     BBa_S04508.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             83..800
                     /label=GFP fusion brick
     misc_feature    83..83
                     /label=deletion of TG
     CDS             83..800
                     /label=BBa_K125500
     stem_loop       820..863
                     /label=stem_loop
     misc_feature    809..888
                     /label=BBa_B0010
     terminator      809..888
                     /label=BBa_B0010
     stem_loop       904..923
                     /label=T7 TE
     misc_feature    930..930
                     /label=stop
     polyA_site      924..937
                     /label=polya
     misc_feature    897..937
                     /label=BBa_B0012
     terminator      897..937
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agcgtaaagg agaagaactt ttcactggag ttgtcccaat
      121 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      181 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      241 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      301 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      361 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      421 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      481 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      541 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      601 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      661 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      721 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      781 tgaactatac aaataataat tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      901 actggctcac cttcgggtgg gcctttctgc gtttata
//