LOCUS BBa_K407007 1530 bp DNA linear 20-Nov-2024 DEFINITION RFP reporter + LuxI ACCESSION BBa_K407007 VERSION BBa_K407007.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..693 /label=mrfp1 misc_feature 700..724 /label=Help:Barcodes CDS 19..724 /label=BBa_E1010 misc_feature 1..724 /label=BBa_K093005 misc_feature 737..740 /label=conserved RBS 733..744 /label=BBa_B0034 misc_feature 1330..1361 /label=LVA CDS 751..1329 /label=luxI misc_feature 751..1368 /label=BBa_C0061 misc_feature 1369..1393 /label=Help:Barcodes CDS 751..1368 /label=BBa_C0061 stem_loop 1413..1456 /label=stem_loop misc_feature 1402..1481 /label=BBa_B0010 terminator 1402..1481 /label=BBa_B0010 stem_loop 1497..1516 /label=T7 TE misc_feature 1523..1523 /label=stop polyA_site 1517..1530 /label=polya misc_feature 1490..1530 /label=BBa_B0012 terminator 1490..1530 /label=BBa_B0012 misc_feature 733..1530 /label=BBa_F1610 ORIGIN 1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc 61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa 121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg 181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa 241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa 301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa 361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg 421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac 481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac 541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa 601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac 661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga 721 tcgctactag agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt 781 ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag 841 caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac 901 tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt 961 ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa 1021 cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat 1081 agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga agctatatat 1141 aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag 1201 cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta 1261 ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca 1321 gtcttaaatg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 1381 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1441 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1501 caccttcggg tgggcctttc tgcgtttata // LOCUS BBa_S04522 1530 bp DNA linear 20-Nov-2024 DEFINITION LuxI with RFP reporter ACCESSION BBa_S04522 VERSION BBa_S04522.1 FEATURES Location/Qualifiers CDS 19..693 /label=mrfp1 misc_feature 700..724 /label=Help:Barcodes CDS 19..699 /label=BBa_E1010 misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..693 /label=mrfp1 misc_feature 700..724 /label=Help:Barcodes CDS 19..724 /label=BBa_E1010 misc_feature 1..724 /label=BBa_K093005 misc_feature 737..740 /label=conserved RBS 733..744 /label=BBa_B0034 misc_feature 1330..1361 /label=LVA CDS 751..1329 /label=luxI misc_feature 751..1368 /label=BBa_C0061 misc_feature 1369..1393 /label=Help:Barcodes CDS 751..1368 /label=BBa_C0061 stem_loop 1413..1456 /label=stem_loop misc_feature 1402..1481 /label=BBa_B0010 terminator 1402..1481 /label=BBa_B0010 stem_loop 1497..1516 /label=T7 TE misc_feature 1523..1523 /label=stop polyA_site 1517..1530 /label=polya misc_feature 1490..1530 /label=BBa_B0012 terminator 1490..1530 /label=BBa_B0012 misc_feature 733..1530 /label=BBa_F1610 ORIGIN 1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc 61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa 121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg 181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa 241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa 301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa 361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg 421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac 481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac 541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa 601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac 661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga 721 tcgctactag agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt 781 ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag 841 caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac 901 tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt 961 ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa 1021 cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat 1081 agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga agctatatat 1141 aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag 1201 cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta 1261 ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca 1321 gtcttaaatg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 1381 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1441 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1501 caccttcggg tgggcctttc tgcgtttata //