LOCUS       BBa_S04523              1808 bp    DNA     linear       20-Nov-2024
DEFINITION  I13507:K329015
ACCESSION   BBa_S04523
VERSION     BBa_S04523.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..693
                     /label=mrfp1
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             19..699
                     /label=BBa_E1010
     stem_loop       744..787
                     /label=stem_loop
     misc_feature    733..812
                     /label=BBa_B0010
     terminator      733..812
                     /label=BBa_B0010
     stem_loop       828..847
                     /label=T7 TE
     misc_feature    854..854
                     /label=stop
     polyA_site      848..861
                     /label=polya
     misc_feature    821..861
                     /label=BBa_B0012
     terminator      821..861
                     /label=BBa_B0012
     misc_feature    1..861
                     /label=BBa_I13507
     misc_feature    870..889
                     /label=LuxR/HSL
     promoter        889..894
                     /label=-35
     promoter        911..916
                     /label=-10
     misc_feature    922..922
                     /label=start
     misc_feature    870..924
                     /label=BBa_R0062
     promoter        870..924
                     /label=BBa_R0062
     RBS             933..945
                     /label=RBS-3\Weak
     RBS             939..942
                     /label=RBS
     misc_feature    933..945
                     /label=BBa_B0032
     RBS             933..945
                     /label=BBa_B0032
     CDS             952..1671
                     /label=GFP protein
     CDS             952..1671
                     /label=BBa_E0040
     stem_loop       1691..1734
                     /label=stem_loop
     misc_feature    1680..1759
                     /label=BBa_B0010
     terminator      1680..1759
                     /label=BBa_B0010
     stem_loop       1775..1794
                     /label=T7 TE
     misc_feature    1801..1801
                     /label=stop
     polyA_site      1795..1808
                     /label=polya
     misc_feature    1768..1808
                     /label=BBa_B0012
     terminator      1768..1808
                     /label=BBa_B0012
     misc_feature    933..1808
                     /label=BBa_E0240
     misc_feature    870..1808
                     /label=BBa_K329015
ORIGIN
        1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
       61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      721 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      841 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
      901 aatggtttgt tatagtcgaa taaatactag agtcacacag gaaagtacta gatgcgtaaa
      961 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     1021 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     1081 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     1141 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
     1201 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     1261 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     1321 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     1381 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     1441 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     1501 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1561 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1621 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
     1681 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1741 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1801 cgtttata
//