##gff-version 3
##sequence-region BBa_S04523 1 1808
BBa_S04523	.	engineered_region	870	1808	.	+	0	ID=BBa_K329015;Name=BBa_K329015
BBa_S04523	.	engineered_region	933	1808	.	+	0	ID=BBa_E0240;Name=BBa_E0240;Parent=BBa_K329015
BBa_S04523	.	terminator	1768	1808	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_S04523	.	stop_codon	1801	1801	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04523	.	polyA_site	1795	1808	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04523	.	stem_loop	1775	1794	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04523	.	engineered_region	1768	1808	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04523	.	terminator	1680	1759	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_S04523	.	engineered_region	1680	1759	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04523	.	stem_loop	1691	1734	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04523	.	ribosome_entry_site	933	945	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_S04523	.	ribosome_entry_site	933	945	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04523	.	engineered_region	933	945	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04523	.	ribosome_entry_site	939	942	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04523	.	CDS	952	1671	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_S04523	.	CDS	952	1671	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04523	.	promoter	870	924	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K329015
BBa_S04523	.	start_codon	922	922	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S04523	.	promoter	911	916	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S04523	.	promoter	889	894	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S04523	.	engineered_region	870	924	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S04523	.	non_covalent_binding_site	870	889	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S04523	.	engineered_region	1	861	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_S04523	.	CDS	19	699	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_S04523	.	engineered_tag	700	724	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04523	.	CDS	19	693	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04523	.	terminator	733	812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_S04523	.	engineered_region	733	812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04523	.	stem_loop	744	787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04523	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_S04523	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04523	.	terminator	821	861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_S04523	.	stop_codon	854	854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04523	.	polyA_site	848	861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04523	.	stem_loop	828	847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04523	.	engineered_region	821	861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S04523
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt
ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa
agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt
aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg
aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg
taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg
gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa
ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa
cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgc
tagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata