LOCUS       BBa_S04540               822 bp    DNA     linear       22-Dec-2024
DEFINITION  C0040:B0015
ACCESSION   BBa_S04540
VERSION     BBa_S04540.1  
FEATURES             Location/Qualifiers
     CDS             4..620
                     /label=tetR
     misc_feature    621..654
                     /label=SsrA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             1..685
                     /label=BBa_C0040
     stem_loop       705..748
                     /label=stem_loop
     misc_feature    694..773
                     /label=BBa_B0010
     terminator      694..773
                     /label=BBa_B0010
     stem_loop       789..808
                     /label=T7 TE
     misc_feature    815..815
                     /label=stop
     polyA_site      809..822
                     /label=polya
     misc_feature    782..822
                     /label=BBa_B0012
     terminator      782..822
                     /label=BBa_B0012
     terminator      694..822
                     /label=BBa_B0015
ORIGIN
        1 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
       61 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
      121 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
      181 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
      241 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
      301 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
      361 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
      421 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
      481 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
      541 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
      601 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa
      661 cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc
      721 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      781 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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