BBa_I13452
1
BBa_I13452
Adds another BioBrick site
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.
false
false
_11_
0
253
6
In stock
false
70 bp from Eco to Spe or Xba to Pst, to allow for gel extraction. The middle portion is largely noise, intended as filler to raise the size of the part.
true
jkm
annotation1891622
1
Future Pst site
range1891622
1
44
49
annotation1891621
1
BstXI site
range1891621
1
41
52
annotation1891620
1
BbsI site
range1891620
1
8
13
annotation1891619
1
Future Eco site
range1891619
1
1
6
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_S04542
1
BBa_S04542
I13452:B0034
2011-04-26T11:00:00Z
2015-05-08T01:14:41Z
false
false
_9_
0
6050
9
Not in stock
false
false
Harland Brandon
component2117831
1
BBa_I13452
component2117833
1
BBa_B0034
annotation2117833
1
BBa_B0034
range2117833
1
61
72
annotation2117831
1
BBa_I13452
range2117831
1
1
52
BBa_B0034_sequence
1
aaagaggagaaa
BBa_S04542_sequence
1
gaattaggtcttcgaatcacaaagattagtcacacgcgatccattgcagtggtactagagaaagaggagaaa
BBa_I13452_sequence
1
gaattaggtcttcgaatcacaaagattagtcacacgcgatccattgcagtgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z