BBa_J70634 1 BBa_J70634 Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment 2010-11-01T12:00:00Z 2015-05-08T01:08:25Z PCR from the M. florum genome. Primers: * ttccgaattcgcggccgcttctagag tccaaatagtgtggatac,Mfl2I-up-F * ttccactagtaaagacatctatgtttttttac,Mfl2I-up-R Approximately 450 bp upstream DNA fragment of M. florum genome from sites 352951-353409 intended as a recombination site for homologous recombination, removing the restriction enzyme and methylase from M. florum. false false _42_41_48_1_ 0 6 48 Not in stock false Avoidance of biobrick restriction sites, possible primer binding sites. false Tom Knight BBa_J70025 1 BBa_J70025 Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid. Upstream promoter for tetM gene; contains a short peptide leader, regulatory sequences which are poorly understood. Use in combination with J70024. false false _41_48_1_ 0 6 48 Not in stock true Alignment of the promoter with the coding region, preservation of the RBS sites. false Tom Knight annotation1999388 1 -10 range1999388 1 68 73 annotation1999384 1 stem_loop range1999384 1 273 320 annotation1999390 1 +1 range1999390 1 80 80 annotation1999385 1 stem_loop range1999385 1 254 281 annotation1999382 1 RBS range1999382 1 339 345 annotation1999383 1 term range1999383 1 319 330 annotation1999386 1 stem_loop range1999386 1 242 272 annotation1999391 1 leader peptide range1999391 1 250 336 annotation1999389 1 -35 range1999389 1 45 50 annotation1999387 1 rbs range1999387 1 237 240 BBa_J70024 1 BBa_J70024 TetM gene, recoded compromise E. coli & Mesoplasma florum 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding. The tetM antibiotic resistance gene synthesized by Geneart from a protein sequence derived from the Ureaplasma urealyticum tetM gene. Synthesized with a compromise codon usage table for expression in both E. coli and Mesoplasma florum. Highly active in both E. coli and Mesoplasma florum. false false _41_48_1_ 0 6 48 Not in stock true Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species. false Tom Knight annotation1982961 1 tetM gene range1982961 1 1 1920 BBa_J70028 1 BBa_J70028 tetM tetracycline resistance cassette 2008-11-04T12:00:00Z 2015-05-08T01:08:20Z Assembled from J70025, J70024 J70025 (pBOT1 tetM promoter upstream region) + J70024 (recoded tetM gene from Geneart plasmid construction) false false _41_48_1_ 0 6 48 Not in stock true Standard assembly false Tom Knight component1999342 1 BBa_J70024 component1999340 1 BBa_J70025 annotation1999342 1 BBa_J70024 range1999342 1 352 2271 annotation1999340 1 BBa_J70025 range1999340 1 1 345 BBa_S04544 1 BBa_S04544 J70634:J70028 2011-06-11T11:00:00Z 2015-05-08T01:14:41Z false false _48_ 0 6 48 Not in stock false false Tom Knight component2120886 1 BBa_J70634 component2120890 1 BBa_J70028 annotation2120886 1 BBa_J70634 range2120886 1 1 459 annotation2120890 1 BBa_J70028 range2120890 1 468 2738 BBa_J70024_sequence 1 atgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa BBa_S04544_sequence 1 tccaaatagtgtggatactgaactacttttaaatgacttgcaagctttaaaagactatcaaccaatcaaggtgccggtttatgattttaaaacttcttcaagaaccaaagaaacaattacagttaaacctagtgatgttgtaatacttgatggaatttttgcactagctattaaagagattagaaaacttggagatatcaaaatttttataaaaacagctaatgatttaaggtttattagacgtttacaaagagatgtaaatgaaagagggagaactttagaaagtgttgttgaacaatacttaaatgaagttaaaccaatgcatgatgcatttattgggccaagtattgaatacgctgatttaataattccttataaagaggggaatgaagttgcagttgatttagtagcaactaaaatattaacattgttaacagagtaaaaaaacatagatgtctttactagagggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaatactagatgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa BBa_J70028_sequence 1 ggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaatactagatgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa BBa_J70025_sequence 1 ggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaa BBa_J70634_sequence 1 tccaaatagtgtggatactgaactacttttaaatgacttgcaagctttaaaagactatcaaccaatcaaggtgccggtttatgattttaaaacttcttcaagaaccaaagaaacaattacagttaaacctagtgatgttgtaatacttgatggaatttttgcactagctattaaagagattagaaaacttggagatatcaaaatttttataaaaacagctaatgatttaaggtttattagacgtttacaaagagatgtaaatgaaagagggagaactttagaaagtgttgttgaacaatacttaaatgaagttaaaccaatgcatgatgcatttattgggccaagtattgaatacgctgatttaataattccttataaagaggggaatgaagttgcagttgatttagtagcaactaaaatattaacattgttaacagagtaaaaaaacatagatgtctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z