BBa_J70028
1
BBa_J70028
tetM tetracycline resistance cassette
2008-11-04T12:00:00Z
2015-05-08T01:08:20Z
Assembled from J70025, J70024
J70025 (pBOT1 tetM promoter upstream region) + J70024 (recoded tetM gene from Geneart plasmid construction)
false
false
_41_48_1_
0
6
48
Not in stock
true
Standard assembly
false
Tom Knight
component1999342
1
BBa_J70024
component1999340
1
BBa_J70025
annotation1999342
1
BBa_J70024
range1999342
1
352
2271
annotation1999340
1
BBa_J70025
range1999340
1
1
345
BBa_J70024
1
BBa_J70024
TetM gene, recoded compromise E. coli & Mesoplasma florum
2008-10-19T11:00:00Z
2015-05-08T01:08:20Z
Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding.
The tetM antibiotic resistance gene synthesized by Geneart from a protein sequence derived from the Ureaplasma urealyticum tetM gene. Synthesized with a compromise codon usage table for expression in both E. coli and Mesoplasma florum. Highly active in both E. coli and Mesoplasma florum.
false
false
_41_48_1_
0
6
48
Not in stock
true
Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species.
false
Tom Knight
annotation1982961
1
tetM gene
range1982961
1
1
1920
BBa_J70634
1
BBa_J70634
Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment
2010-11-01T12:00:00Z
2015-05-08T01:08:25Z
PCR from the M. florum genome.
Primers:
* ttccgaattcgcggccgcttctagag tccaaatagtgtggatac,Mfl2I-up-F
* ttccactagtaaagacatctatgtttttttac,Mfl2I-up-R
Approximately 450 bp upstream DNA fragment of M. florum genome from sites 352951-353409 intended as a recombination site for homologous recombination, removing the restriction enzyme and methylase from M. florum.
false
false
_42_41_48_1_
0
6
48
Not in stock
false
Avoidance of biobrick restriction sites, possible primer binding sites.
false
Tom Knight
BBa_S04544
1
BBa_S04544
J70634:J70028
2011-06-11T11:00:00Z
2015-05-08T01:14:41Z
false
false
_48_
0
6
48
Not in stock
false
false
Tom Knight
component2120890
1
BBa_J70028
component2120886
1
BBa_J70634
annotation2120890
1
BBa_J70028
range2120890
1
468
2738
annotation2120886
1
BBa_J70634
range2120886
1
1
459
BBa_J70025
1
BBa_J70025
Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1
2008-10-19T11:00:00Z
2015-05-08T01:08:20Z
pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid.
Upstream promoter for tetM gene; contains a short peptide leader, regulatory sequences which are poorly understood. Use in combination with J70024.
false
false
_41_48_1_
0
6
48
Not in stock
true
Alignment of the promoter with the coding region, preservation of the RBS sites.
false
Tom Knight
annotation1999382
1
RBS
range1999382
1
339
345
annotation1999387
1
rbs
range1999387
1
237
240
annotation1999388
1
-10
range1999388
1
68
73
annotation1999385
1
stem_loop
range1999385
1
254
281
annotation1999383
1
term
range1999383
1
319
330
annotation1999384
1
stem_loop
range1999384
1
273
320
annotation1999390
1
+1
range1999390
1
80
80
annotation1999386
1
stem_loop
range1999386
1
242
272
annotation1999391
1
leader peptide
range1999391
1
250
336
annotation1999389
1
-35
range1999389
1
45
50
BBa_J70024_sequence
1
atgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa
BBa_S04544_sequence
1
tccaaatagtgtggatactgaactacttttaaatgacttgcaagctttaaaagactatcaaccaatcaaggtgccggtttatgattttaaaacttcttcaagaaccaaagaaacaattacagttaaacctagtgatgttgtaatacttgatggaatttttgcactagctattaaagagattagaaaacttggagatatcaaaatttttataaaaacagctaatgatttaaggtttattagacgtttacaaagagatgtaaatgaaagagggagaactttagaaagtgttgttgaacaatacttaaatgaagttaaaccaatgcatgatgcatttattgggccaagtattgaatacgctgatttaataattccttataaagaggggaatgaagttgcagttgatttagtagcaactaaaatattaacattgttaacagagtaaaaaaacatagatgtctttactagagggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaatactagatgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa
BBa_J70028_sequence
1
ggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaatactagatgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa
BBa_J70025_sequence
1
ggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaa
BBa_J70634_sequence
1
tccaaatagtgtggatactgaactacttttaaatgacttgcaagctttaaaagactatcaaccaatcaaggtgccggtttatgattttaaaacttcttcaagaaccaaagaaacaattacagttaaacctagtgatgttgtaatacttgatggaatttttgcactagctattaaagagattagaaaacttggagatatcaaaatttttataaaaacagctaatgatttaaggtttattagacgtttacaaagagatgtaaatgaaagagggagaactttagaaagtgttgttgaacaatacttaaatgaagttaaaccaatgcatgatgcatttattgggccaagtattgaatacgctgatttaataattccttataaagaggggaatgaagttgcagttgatttagtagcaactaaaatattaacattgttaacagagtaaaaaaacatagatgtctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z