LOCUS       BBa_S04545              2980 bp    DNA     linear       22-Jun-2024
DEFINITION  J70028:J70635
ACCESSION   BBa_S04545
VERSION     BBa_S04545.1  
FEATURES             Location/Qualifiers
     RBS             339..345
                     /label=RBS
     stem_loop       319..330
                     /label=term
     stem_loop       273..320
                     /label=stem_loop
     stem_loop       254..281
                     /label=stem_loop
     stem_loop       242..272
                     /label=stem_loop
     RBS             237..240
                     /label=rbs
     promoter        68..73
                     /label=-10
     promoter        45..50
                     /label=-35
     misc_feature    80..80
                     /label=+1
     CDS             250..336
                     /label=leader peptide
     promoter        1..345
                     /label=BBa_J70025
     CDS             352..2271
                     /label=tetM gene
     CDS             352..2271
                     /label=BBa_J70024
     misc_feature    1..2271
                     /label=BBa_J70028
     misc_feature    2280..2980
                     /label=BBa_J70635
ORIGIN
        1 ggccagtcta catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct
       61 tacgtgctat tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc
      121 ttaaataaac tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg
      181 cttagttttt tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag
      241 aatatctgta tgctttgtat acctatggtt atgcataaaa atcccagtga taaaagtatt
      301 tatcactggg atttttatgc ccttttgggt ttttgaatgg aggaatacta gatgaaaatc
      361 ataaatatcg gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt
      421 ttatataact ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact
      481 gataatacat tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt
      541 caatgggaaa atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca
      601 gaagtatacc gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat
      661 ggagttcaag ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca
      721 attttcttta taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat
      781 atcaaagaaa aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac
      841 gtttgcgtaa caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat
      901 gatttattag aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa
      961 gaaagtattc gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt
     1021 aacataggta ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat
     1081 cgtggtcctt ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa
     1141 cgtttagctt atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt
     1201 agtgaaaaag aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc
     1261 aaaattgacc gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt
     1321 aatagtgtac ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac
     1381 ccattacttc aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat
     1441 gctcttttag aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact
     1501 catgaaatta ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta
     1561 caagaaaaat atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt
     1621 ccattaaaaa atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct
     1681 tctattggtc tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc
     1741 gtaagtttag gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac
     1801 ggttgcgaac aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat
     1861 ggactttact attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt
     1921 ttagaacaag ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa
     1981 atctatgcac cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct
     2041 aatattgttg atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca
     2101 cgttgtattc aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc
     2161 ttaactgaat taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt
     2221 cctaatagtc gtattgataa agttcgttat atgttcaaca aaatcacata atactagagg
     2281 agattattat ggcaaaagaa gtaaaatatg atgaatcggc cattcaggtc cttgaaggtt
     2341 tagaagctgt tagaaaacgt ccagggatgt atattggttc aactgatgtt agaggactac
     2401 accacttagt atgagaaatt gtagataact caatcgatga agctttagca ggatattgta
     2461 cagaaattaa cgtaacttta gaaaaaaatg gaagtgttac tgtcgctgat aatggtcgtg
     2521 gagttccaat tgggatgcac tcaacaggaa gaccaacacc tgaagttatt tttagtgtac
     2581 ttcatgctgg aggaaaattt ggtggaagtg gttataaaac atctggagga cttcacggag
     2641 ttgggtcatc tgttgttaat gctttatcta aaaaatttaa tgttacaatt tatcgtgata
     2701 aaaaagttaa tgaaattgaa tttactaatg gaggaaaatt aaatattcct ttaacagaag
     2761 ttggaacaac taacaaaact ggtactgttg ttaacttctt accagatgat tcaattttta
     2821 atactacaaa atttaacttc acaacaatta gtgaaagatt aaaagaatca gctttactaa
     2881 actcaggttt aaaaattaca atatcagaca aagttaatga taagtttgtt gaatatcaat
     2941 ttgaaaatgg tttagtagag tttgttaagg aattatcaag
//