LOCUS       BBa_S04546              3447 bp    DNA     linear       15-Nov-2024
DEFINITION  J70634:S04545
ACCESSION   BBa_S04546
VERSION     BBa_S04546.1  
FEATURES             Location/Qualifiers
     misc_feature    1..459
                     /label=BBa_J70634
     RBS             806..812
                     /label=RBS
     stem_loop       786..797
                     /label=term
     stem_loop       740..787
                     /label=stem_loop
     stem_loop       721..748
                     /label=stem_loop
     stem_loop       709..739
                     /label=stem_loop
     RBS             704..707
                     /label=rbs
     promoter        535..540
                     /label=-10
     promoter        512..517
                     /label=-35
     misc_feature    547..547
                     /label=+1
     CDS             717..803
                     /label=leader peptide
     promoter        468..812
                     /label=BBa_J70025
     CDS             819..2738
                     /label=tetM gene
     CDS             819..2738
                     /label=BBa_J70024
     misc_feature    468..2738
                     /label=BBa_J70028
     misc_feature    2747..3447
                     /label=BBa_J70635
     misc_feature    468..3447
                     /label=BBa_S04545
ORIGIN
        1 tccaaatagt gtggatactg aactactttt aaatgacttg caagctttaa aagactatca
       61 accaatcaag gtgccggttt atgattttaa aacttcttca agaaccaaag aaacaattac
      121 agttaaacct agtgatgttg taatacttga tggaattttt gcactagcta ttaaagagat
      181 tagaaaactt ggagatatca aaatttttat aaaaacagct aatgatttaa ggtttattag
      241 acgtttacaa agagatgtaa atgaaagagg gagaacttta gaaagtgttg ttgaacaata
      301 cttaaatgaa gttaaaccaa tgcatgatgc atttattggg ccaagtattg aatacgctga
      361 tttaataatt ccttataaag aggggaatga agttgcagtt gatttagtag caactaaaat
      421 attaacattg ttaacagagt aaaaaaacat agatgtcttt actagagggc cagtctacat
      481 gtactctttt tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat
      541 ttaagtgact atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt
      601 caatttgata gcgggaacaa ataattagat gtcctttttt aggagggctt agttttttgt
      661 acccagttta agaatacctt tatcatgtga ttctaaagta tccagagaat atctgtatgc
      721 tttgtatacc tatggttatg cataaaaatc ccagtgataa aagtatttat cactgggatt
      781 tttatgccct tttgggtttt tgaatggagg aatactagat gaaaatcata aatatcggtg
      841 tattagctca cgttgatgca ggaaaaacaa cattaactga atcactttta tataactctg
      901 gtgcaattac tgaacttggt tcagtagata aaggtactac tcgtactgat aatacattat
      961 tagaacgtca acgtggaatc acaattcaaa caggtatcac atcttttcaa tgggaaaata
     1021 caaaagtaaa tattatagat acacctggac acatggattt ccttgcagaa gtataccgta
     1081 gtctttcagt attagatggt gctattttac ttatcagcgc taaagatgga gttcaagctc
     1141 aaactcgtat cttatttcac gcattacgta aaatgggtat tccaacaatt ttctttataa
     1201 acaaaattga ccaaaacgga attgatttaa gtacagttta tcaagatatc aaagaaaaac
     1261 tttctgctga aatcgttatt aaacaaaaag ttgaattata cccaaacgtt tgcgtaacaa
     1321 attttactga atcagaacaa tgggatacag ttatagaagg taatgatgat ttattagaaa
     1381 aatacatgtc aggtaaatca ttagaagcat tagaattaga acaagaagaa agtattcgtt
     1441 tccaaaactg ttctttattc cctttatacc atggaagcgc taaaagtaac ataggtattg
     1501 ataacttaat tgaagttatt actaacaaat tttactcttc aactcatcgt ggtccttctg
     1561 aattatgcgg taacgttttc aaaattgaat atacaaaaaa acgtcaacgt ttagcttata
     1621 tacgtcttta tagtggtgtt ttacatttac gtgatagtgt tcgtgttagt gaaaaagaaa
     1681 agattaaagt tacagaaatg tatacttcta ttaacggtga attatgcaaa attgaccgtg
     1741 catattcagg tgaaattgta attttacaaa acgaatttct taaacttaat agtgtacttg
     1801 gtgacacaaa acttttacca caacgtaaga aaattgaaaa tccacaccca ttacttcaaa
     1861 caacagtaga accaagcaaa cctgaacaac gtgaaatgct tttagatgct cttttagaaa
     1921 ttagtgactc tgacccactt ttacgttact atgtagattc tactactcat gaaattattc
     1981 tttctttcct tggtaaagtt caaatggaag ttatttctgc attattacaa gaaaaatatc
     2041 atgttgaaat cgaattaaaa gaacctactg taatttatat ggaacgtcca ttaaaaaatg
     2101 ctgaatatac aattcatatt gaagttccac caaatccatt ttgggcttct attggtcttt
     2161 ctgtttctcc acttccactt ggtagcggaa tgcaatatga aagtagcgta agtttaggtt
     2221 atcttaatca aagtttccaa aacgcagtta tggaaggtat tcgttacggt tgcgaacaag
     2281 gtttatacgg ttggaatgtt acagactgca aaatctgttt taagtatgga ctttactatt
     2341 cacctgtatc aacacctgct gactttcgta tgcttgcacc aattgtttta gaacaagttt
     2401 taaagaaagc tggaactgaa cttttagaac catacctttc ttttaaaatc tatgcaccac
     2461 aagaatactt aagtcgtgct tataacgatg cacctaaata ctgtgctaat attgttgata
     2521 ctcaattaaa gaacaacgaa gtaattttaa gcggagaaat tcctgcacgt tgtattcaag
     2581 aatatcgtag tgatttaaca tttttcacta atggacgttc tgtttgctta actgaattaa
     2641 aaggttatca tgttactact ggtgaacctg tatgccaacc acgtcgtcct aatagtcgta
     2701 ttgataaagt tcgttatatg ttcaacaaaa tcacataata ctagaggaga ttattatggc
     2761 aaaagaagta aaatatgatg aatcggccat tcaggtcctt gaaggtttag aagctgttag
     2821 aaaacgtcca gggatgtata ttggttcaac tgatgttaga ggactacacc acttagtatg
     2881 agaaattgta gataactcaa tcgatgaagc tttagcagga tattgtacag aaattaacgt
     2941 aactttagaa aaaaatggaa gtgttactgt cgctgataat ggtcgtggag ttccaattgg
     3001 gatgcactca acaggaagac caacacctga agttattttt agtgtacttc atgctggagg
     3061 aaaatttggt ggaagtggtt ataaaacatc tggaggactt cacggagttg ggtcatctgt
     3121 tgttaatgct ttatctaaaa aatttaatgt tacaatttat cgtgataaaa aagttaatga
     3181 aattgaattt actaatggag gaaaattaaa tattccttta acagaagttg gaacaactaa
     3241 caaaactggt actgttgtta acttcttacc agatgattca atttttaata ctacaaaatt
     3301 taacttcaca acaattagtg aaagattaaa agaatcagct ttactaaact caggtttaaa
     3361 aattacaata tcagacaaag ttaatgataa gtttgttgaa tatcaatttg aaaatggttt
     3421 agtagagttt gttaaggaat tatcaag
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