##gff-version 3
##sequence-region BBa_S04547 1 2470
BBa_S04547	.	engineered_region	864	2470	.	+	0	ID=BBa_K116634;Name=BBa_K116634
BBa_S04547	.	engineered_region	864	1663	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_S04547	.	CDS	883	1638	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04547	.	point_mutation	1374	1374	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04547	.	CDS	883	1632	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04547	.	point_mutation	1056	1056	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04547	.	engineered_tag	1639	1663	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04547	.	engineered_region	883	1638	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04547	.	engineered_region	883	884	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04547	.	ribosome_entry_site	864	876	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04547	.	ribosome_entry_site	870	873	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04547	.	ribosome_entry_site	864	876	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04547	.	engineered_region	864	876	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04547	.	engineered_region	1672	2470	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_S04547	.	ribosome_entry_site	1672	1684	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_S04547	.	ribosome_entry_site	1678	1681	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04547	.	ribosome_entry_site	1672	1684	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04547	.	engineered_region	1672	1684	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04547	.	engineered_region	1691	2470	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_S04547	.	CDS	1691	2308	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_S04547	.	CDS	1691	2269	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04547	.	engineered_tag	2309	2333	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04547	.	engineered_region	1691	2308	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04547	.	tag	2270	2301	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04547	.	terminator	2342	2470	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_S04547	.	terminator	2430	2470	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04547	.	polyA_site	2457	2470	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04547	.	stem_loop	2437	2456	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04547	.	engineered_region	2430	2470	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04547	.	stop_codon	2463	2463	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04547	.	terminator	2342	2421	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04547	.	stem_loop	2353	2396	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04547	.	engineered_region	2342	2421	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04547	.	engineered_region	1	855	.	+	0	ID=BBa_K208016;Name=BBa_K208016
BBa_S04547	.	terminator	815	855	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208016
BBa_S04547	.	polyA_site	842	855	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04547	.	stem_loop	822	841	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04547	.	engineered_region	815	855	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04547	.	stop_codon	848	848	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04547	.	CDS	1	718	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K208016
BBa_S04547	.	CDS	1	718	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_S04547	.	point_mutation	1	1	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_S04547	.	terminator	727	806	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208016
BBa_S04547	.	stem_loop	738	781	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04547	.	engineered_region	727	806	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S04547
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatttt
ctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacc
tgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacag
catgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactaca
agacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaaga
tggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaat
ggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaata
ctccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaa
cgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa
taataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacac
aggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaat
aatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctc
attctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaattt
aataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgct
gtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcata
cggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgc
gtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaac
aacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatat
taggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaag
tatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtaga
tcactactagagtcacacaggaaagtactagatgactataatgataaaaaaatcggattttttggcaattccatcggag
gagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataacc
ttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctg
gcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaa
gatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaatta
caatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagc
aatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatact
aaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacg
ctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata