LOCUS       BBa_S04548              2493 bp    DNA     linear       22-Dec-2024
DEFINITION  B0030:S04547
ACCESSION   BBa_S04548
VERSION     BBa_S04548.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             24..741
                     /label=GFP fusion brick
     misc_feature    24..24
                     /label=deletion of TG
     CDS             24..741
                     /label=BBa_K125500
     stem_loop       761..804
                     /label=stem_loop
     misc_feature    750..829
                     /label=BBa_B0010
     terminator      750..829
                     /label=BBa_B0010
     stem_loop       845..864
                     /label=T7 TE
     misc_feature    871..871
                     /label=stop
     polyA_site      865..878
                     /label=polya
     misc_feature    838..878
                     /label=BBa_B0012
     terminator      838..878
                     /label=BBa_B0012
     misc_feature    24..878
                     /label=BBa_K208016
     RBS             887..899
                     /label=RBS-3\Weak
     RBS             893..896
                     /label=RBS
     misc_feature    887..899
                     /label=BBa_B0032
     RBS             887..899
                     /label=BBa_B0032
     misc_feature    906..907
                     /label=prefix
     misc_feature    1079..1079
                     /label=T
     misc_feature    1397..1397
                     /label=A
     CDS             906..1655
                     /label=luxR
     misc_feature    906..1661
                     /label=BBa_C0062
     misc_feature    1662..1686
                     /label=Help:Barcodes
     CDS             906..1661
                     /label=BBa_C0062
     misc_feature    887..1686
                     /label=BBa_K116616
     RBS             1695..1707
                     /label=RBS-3\Weak
     RBS             1701..1704
                     /label=RBS
     misc_feature    1695..1707
                     /label=BBa_B0032
     RBS             1695..1707
                     /label=BBa_B0032
     misc_feature    2293..2324
                     /label=LVA
     CDS             1714..2292
                     /label=luxI
     misc_feature    1714..2331
                     /label=BBa_C0061
     misc_feature    2332..2356
                     /label=Help:Barcodes
     CDS             1714..2331
                     /label=BBa_C0061
     stem_loop       2376..2419
                     /label=stem_loop
     misc_feature    2365..2444
                     /label=BBa_B0010
     terminator      2365..2444
                     /label=BBa_B0010
     stem_loop       2460..2479
                     /label=T7 TE
     misc_feature    2486..2486
                     /label=stop
     polyA_site      2480..2493
                     /label=polya
     misc_feature    2453..2493
                     /label=BBa_B0012
     terminator      2453..2493
                     /label=BBa_B0012
     terminator      2365..2493
                     /label=BBa_B0015
     misc_feature    1714..2493
                     /label=BBa_K116615
     misc_feature    1695..2493
                     /label=BBa_K116625
     misc_feature    887..2493
                     /label=BBa_K116634
     misc_feature    24..2493
                     /label=BBa_S04547
ORIGIN
        1 attaaagagg agaaatacta gagcgtaaag gagaagaact tttcactgga gttgtcccaa
       61 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      121 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      181 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      241 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      301 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      361 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      421 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      481 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      541 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      601 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      661 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      721 atgaactata caaataataa ttactagagc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtcac acaggaaagt
      901 actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt
      961 gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg
     1021 aatattattt actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc
     1081 tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg
     1141 atcctatagt agattattct aactccaatc attcaccaat taattggaat atatttgaaa
     1201 acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca tcaggtctta
     1261 tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg cttagttttg
     1321 cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt atgaacatac
     1381 cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca aataataaat
     1441 caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa
     1501 gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact ttccatttaa
     1561 ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct aaagcaattt
     1621 taacaggagc aattgattgc ccatacttta aaaattaata acactgatag tgctagtgta
     1681 gatcactact agagtcacac aggaaagtac tagatgacta taatgataaa aaaatcggat
     1741 tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt
     1801 aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat
     1861 aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg
     1921 cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt
     1981 caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa
     2041 aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata
     2101 tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata
     2161 gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat
     2221 gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa
     2281 gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag
     2341 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2401 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2461 gctcaccttc gggtgggcct ttctgcgttt ata
//