LOCUS BBa_S04548 2493 bp DNA linear 22-Nov-2024 DEFINITION B0030:S04547 ACCESSION BBa_S04548 VERSION BBa_S04548.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS-1\Strong RBS 8..12 /label=RBS misc_feature 1..15 /label=BBa_B0030 RBS 1..15 /label=BBa_B0030 CDS 24..741 /label=GFP fusion brick misc_feature 24..24 /label=deletion of TG CDS 24..741 /label=BBa_K125500 stem_loop 761..804 /label=stem_loop misc_feature 750..829 /label=BBa_B0010 terminator 750..829 /label=BBa_B0010 stem_loop 845..864 /label=T7 TE misc_feature 871..871 /label=stop polyA_site 865..878 /label=polya misc_feature 838..878 /label=BBa_B0012 terminator 838..878 /label=BBa_B0012 misc_feature 24..878 /label=BBa_K208016 RBS 887..899 /label=RBS-3\Weak RBS 893..896 /label=RBS misc_feature 887..899 /label=BBa_B0032 RBS 887..899 /label=BBa_B0032 misc_feature 906..907 /label=prefix misc_feature 1079..1079 /label=T misc_feature 1397..1397 /label=A CDS 906..1655 /label=luxR misc_feature 906..1661 /label=BBa_C0062 misc_feature 1662..1686 /label=Help:Barcodes CDS 906..1661 /label=BBa_C0062 misc_feature 887..1686 /label=BBa_K116616 RBS 1695..1707 /label=RBS-3\Weak RBS 1701..1704 /label=RBS misc_feature 1695..1707 /label=BBa_B0032 RBS 1695..1707 /label=BBa_B0032 misc_feature 2293..2324 /label=LVA CDS 1714..2292 /label=luxI misc_feature 1714..2331 /label=BBa_C0061 misc_feature 2332..2356 /label=Help:Barcodes CDS 1714..2331 /label=BBa_C0061 stem_loop 2376..2419 /label=stem_loop misc_feature 2365..2444 /label=BBa_B0010 terminator 2365..2444 /label=BBa_B0010 stem_loop 2460..2479 /label=T7 TE misc_feature 2486..2486 /label=stop polyA_site 2480..2493 /label=polya misc_feature 2453..2493 /label=BBa_B0012 terminator 2453..2493 /label=BBa_B0012 terminator 2365..2493 /label=BBa_B0015 misc_feature 1714..2493 /label=BBa_K116615 misc_feature 1695..2493 /label=BBa_K116625 misc_feature 887..2493 /label=BBa_K116634 misc_feature 24..2493 /label=BBa_S04547 ORIGIN 1 attaaagagg agaaatacta gagcgtaaag gagaagaact tttcactgga gttgtcccaa 61 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 121 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 181 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 241 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 301 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 361 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 421 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 481 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 541 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 601 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 661 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 721 atgaactata caaataataa ttactagagc caggcatcaa ataaaacgaa aggctcagtc 781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtcac acaggaaagt 901 actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt 961 gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg 1021 aatattattt actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc 1081 tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg 1141 atcctatagt agattattct aactccaatc attcaccaat taattggaat atatttgaaa 1201 acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca tcaggtctta 1261 tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg cttagttttg 1321 cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt atgaacatac 1381 cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca aataataaat 1441 caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa 1501 gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact ttccatttaa 1561 ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct aaagcaattt 1621 taacaggagc aattgattgc ccatacttta aaaattaata acactgatag tgctagtgta 1681 gatcactact agagtcacac aggaaagtac tagatgacta taatgataaa aaaatcggat 1741 tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt 1801 aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat 1861 aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg 1921 cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt 1981 caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa 2041 aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata 2101 tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata 2161 gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat 2221 gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa 2281 gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 2341 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 2401 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 2461 gctcaccttc gggtgggcct ttctgcgttt ata //