##gff-version 3
##sequence-region BBa_S04548 1 2493
BBa_S04548	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S04548	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04548	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04548	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04548	.	engineered_region	24	2493	.	+	0	ID=BBa_S04547;Name=BBa_S04547
BBa_S04548	.	engineered_region	887	2493	.	+	0	ID=BBa_K116634;Name=BBa_K116634;Parent=BBa_S04547
BBa_S04548	.	engineered_region	1695	2493	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_S04548	.	engineered_region	1714	2493	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_S04548	.	terminator	2365	2493	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_S04548	.	terminator	2365	2444	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04548	.	stem_loop	2376	2419	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04548	.	engineered_region	2365	2444	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04548	.	terminator	2453	2493	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04548	.	polyA_site	2480	2493	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04548	.	engineered_region	2453	2493	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04548	.	stop_codon	2486	2486	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04548	.	stem_loop	2460	2479	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04548	.	CDS	1714	2331	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_S04548	.	engineered_tag	2332	2356	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04548	.	CDS	1714	2292	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04548	.	engineered_region	1714	2331	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04548	.	tag	2293	2324	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04548	.	ribosome_entry_site	1695	1707	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_S04548	.	ribosome_entry_site	1695	1707	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04548	.	engineered_region	1695	1707	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04548	.	ribosome_entry_site	1701	1704	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04548	.	engineered_region	887	1686	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_S04548	.	CDS	906	1661	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04548	.	point_mutation	1397	1397	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04548	.	engineered_tag	1662	1686	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04548	.	engineered_region	906	1661	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04548	.	CDS	906	1655	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04548	.	point_mutation	1079	1079	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04548	.	engineered_region	906	907	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04548	.	ribosome_entry_site	887	899	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04548	.	ribosome_entry_site	887	899	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04548	.	engineered_region	887	899	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04548	.	ribosome_entry_site	893	896	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04548	.	engineered_region	24	878	.	+	0	ID=BBa_K208016;Name=BBa_K208016;Parent=BBa_S04547
BBa_S04548	.	CDS	24	741	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K208016
BBa_S04548	.	CDS	24	741	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_S04548	.	point_mutation	24	24	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_S04548	.	terminator	750	829	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208016
BBa_S04548	.	stem_loop	761	804	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04548	.	engineered_region	750	829	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04548	.	terminator	838	878	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208016
BBa_S04548	.	polyA_site	865	878	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04548	.	engineered_region	838	878	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04548	.	stop_codon	871	871	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04548	.	stem_loop	845	864	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_S04548
attaaagaggagaaatactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatgg
tgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattt
atttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcga
gatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatt
tttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtta
aaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtataca
tcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaact
agcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaa
tctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacac
atggcatggatgaactatacaaataataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaata
aaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatatta
tttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggagg
caatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaatt
ggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcac
tgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactat
atagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataa
atatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaag
ctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactc
aatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaat
aacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgactataatgataaaaaaatcgg
attttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtg
ggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgat
actgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgc
ttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagat
aaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaa
tatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaag
aaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaa
tgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata