LOCUS       BBa_S04549              2492 bp    DNA     linear       05-Nov-2024
DEFINITION  B0031:S04547
ACCESSION   BBa_S04549
VERSION     BBa_S04549.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             23..740
                     /label=GFP fusion brick
     misc_feature    23..23
                     /label=deletion of TG
     CDS             23..740
                     /label=BBa_K125500
     stem_loop       760..803
                     /label=stem_loop
     misc_feature    749..828
                     /label=BBa_B0010
     terminator      749..828
                     /label=BBa_B0010
     stem_loop       844..863
                     /label=T7 TE
     misc_feature    870..870
                     /label=stop
     polyA_site      864..877
                     /label=polya
     misc_feature    837..877
                     /label=BBa_B0012
     terminator      837..877
                     /label=BBa_B0012
     misc_feature    23..877
                     /label=BBa_K208016
     RBS             886..898
                     /label=RBS-3\Weak
     RBS             892..895
                     /label=RBS
     misc_feature    886..898
                     /label=BBa_B0032
     RBS             886..898
                     /label=BBa_B0032
     misc_feature    905..906
                     /label=prefix
     misc_feature    1078..1078
                     /label=T
     misc_feature    1396..1396
                     /label=A
     CDS             905..1654
                     /label=luxR
     misc_feature    905..1660
                     /label=BBa_C0062
     misc_feature    1661..1685
                     /label=Help:Barcodes
     CDS             905..1660
                     /label=BBa_C0062
     misc_feature    886..1685
                     /label=BBa_K116616
     RBS             1694..1706
                     /label=RBS-3\Weak
     RBS             1700..1703
                     /label=RBS
     misc_feature    1694..1706
                     /label=BBa_B0032
     RBS             1694..1706
                     /label=BBa_B0032
     misc_feature    2292..2323
                     /label=LVA
     CDS             1713..2291
                     /label=luxI
     misc_feature    1713..2330
                     /label=BBa_C0061
     misc_feature    2331..2355
                     /label=Help:Barcodes
     CDS             1713..2330
                     /label=BBa_C0061
     stem_loop       2375..2418
                     /label=stem_loop
     misc_feature    2364..2443
                     /label=BBa_B0010
     terminator      2364..2443
                     /label=BBa_B0010
     stem_loop       2459..2478
                     /label=T7 TE
     misc_feature    2485..2485
                     /label=stop
     polyA_site      2479..2492
                     /label=polya
     misc_feature    2452..2492
                     /label=BBa_B0012
     terminator      2452..2492
                     /label=BBa_B0012
     terminator      2364..2492
                     /label=BBa_B0015
     misc_feature    1713..2492
                     /label=BBa_K116615
     misc_feature    1694..2492
                     /label=BBa_K116625
     misc_feature    886..2492
                     /label=BBa_K116634
     misc_feature    23..2492
                     /label=BBa_S04547
ORIGIN
        1 tcacacagga aacctactag agcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataataat tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      781 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      841 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtcaca caggaaagta
      901 ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg
      961 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga
     1021 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct
     1081 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga
     1141 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa
     1201 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat
     1261 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc
     1321 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc
     1381 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc
     1441 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag
     1501 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac
     1561 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt
     1621 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag
     1681 atcactacta gagtcacaca ggaaagtact agatgactat aatgataaaa aaatcggatt
     1741 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta
     1801 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata
     1861 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc
     1921 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc
     1981 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa
     2041 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat
     2101 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag
     2161 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg
     2221 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag
     2281 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     2341 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2401 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2461 ctcaccttcg ggtgggcctt tctgcgttta ta
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