##gff-version 3
##sequence-region BBa_S04549 1 2492
BBa_S04549	.	engineered_region	23	2492	.	+	0	ID=BBa_S04547;Name=BBa_S04547
BBa_S04549	.	engineered_region	23	877	.	+	0	ID=BBa_K208016;Name=BBa_K208016;Parent=BBa_S04547
BBa_S04549	.	CDS	23	740	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K208016
BBa_S04549	.	point_mutation	23	23	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_S04549	.	CDS	23	740	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_S04549	.	terminator	837	877	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208016
BBa_S04549	.	polyA_site	864	877	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04549	.	engineered_region	837	877	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04549	.	stem_loop	844	863	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04549	.	stop_codon	870	870	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04549	.	terminator	749	828	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208016
BBa_S04549	.	stem_loop	760	803	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04549	.	engineered_region	749	828	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04549	.	engineered_region	886	2492	.	+	0	ID=BBa_K116634;Name=BBa_K116634;Parent=BBa_S04547
BBa_S04549	.	engineered_region	886	1685	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_S04549	.	ribosome_entry_site	886	898	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04549	.	engineered_region	886	898	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04549	.	ribosome_entry_site	886	898	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04549	.	ribosome_entry_site	892	895	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04549	.	CDS	905	1660	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04549	.	engineered_tag	1661	1685	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04549	.	engineered_region	905	1660	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04549	.	CDS	905	1654	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04549	.	point_mutation	1078	1078	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04549	.	point_mutation	1396	1396	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04549	.	engineered_region	905	906	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04549	.	engineered_region	1694	2492	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_S04549	.	ribosome_entry_site	1694	1706	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_S04549	.	engineered_region	1694	1706	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04549	.	ribosome_entry_site	1694	1706	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04549	.	ribosome_entry_site	1700	1703	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04549	.	engineered_region	1713	2492	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_S04549	.	CDS	1713	2330	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_S04549	.	engineered_region	1713	2330	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04549	.	tag	2292	2323	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04549	.	engineered_tag	2331	2355	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04549	.	CDS	1713	2291	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04549	.	terminator	2364	2492	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_S04549	.	terminator	2452	2492	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04549	.	polyA_site	2479	2492	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04549	.	engineered_region	2452	2492	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04549	.	stem_loop	2459	2478	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04549	.	stop_codon	2485	2485	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04549	.	terminator	2364	2443	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04549	.	stem_loop	2375	2418	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04549	.	engineered_region	2364	2443	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04549	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_S04549	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
>BBa_S04549
tcacacaggaaacctactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggt
gatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgag
atacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattt
ttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaa
aaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacat
catggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaacta
gcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaat
ctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacaca
tggcatggatgaactatacaaataataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataa
aattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattat
ttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggc
aatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattg
gaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcact
gggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactata
tagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaa
tatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagc
tcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactca
atacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaata
acactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgactataatgataaaaaaatcgga
ttttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgg
gacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgata
ctgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgct
tggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagata
aataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaat
atgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaaga
aattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaat
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata