LOCUS       BBa_S04551              2491 bp    DNA     linear       05-Nov-2024
DEFINITION  B0032:S04547
ACCESSION   BBa_S04551
VERSION     BBa_S04551.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             22..739
                     /label=GFP fusion brick
     misc_feature    22..22
                     /label=deletion of TG
     CDS             22..739
                     /label=BBa_K125500
     stem_loop       759..802
                     /label=stem_loop
     misc_feature    748..827
                     /label=BBa_B0010
     terminator      748..827
                     /label=BBa_B0010
     stem_loop       843..862
                     /label=T7 TE
     misc_feature    869..869
                     /label=stop
     polyA_site      863..876
                     /label=polya
     misc_feature    836..876
                     /label=BBa_B0012
     terminator      836..876
                     /label=BBa_B0012
     misc_feature    22..876
                     /label=BBa_K208016
     RBS             885..897
                     /label=RBS-3\Weak
     RBS             891..894
                     /label=RBS
     misc_feature    885..897
                     /label=BBa_B0032
     RBS             885..897
                     /label=BBa_B0032
     misc_feature    904..905
                     /label=prefix
     misc_feature    1077..1077
                     /label=T
     misc_feature    1395..1395
                     /label=A
     CDS             904..1653
                     /label=luxR
     misc_feature    904..1659
                     /label=BBa_C0062
     misc_feature    1660..1684
                     /label=Help:Barcodes
     CDS             904..1659
                     /label=BBa_C0062
     misc_feature    885..1684
                     /label=BBa_K116616
     RBS             1693..1705
                     /label=RBS-3\Weak
     RBS             1699..1702
                     /label=RBS
     misc_feature    1693..1705
                     /label=BBa_B0032
     RBS             1693..1705
                     /label=BBa_B0032
     misc_feature    2291..2322
                     /label=LVA
     CDS             1712..2290
                     /label=luxI
     misc_feature    1712..2329
                     /label=BBa_C0061
     misc_feature    2330..2354
                     /label=Help:Barcodes
     CDS             1712..2329
                     /label=BBa_C0061
     stem_loop       2374..2417
                     /label=stem_loop
     misc_feature    2363..2442
                     /label=BBa_B0010
     terminator      2363..2442
                     /label=BBa_B0010
     stem_loop       2458..2477
                     /label=T7 TE
     misc_feature    2484..2484
                     /label=stop
     polyA_site      2478..2491
                     /label=polya
     misc_feature    2451..2491
                     /label=BBa_B0012
     terminator      2451..2491
                     /label=BBa_B0012
     terminator      2363..2491
                     /label=BBa_B0015
     misc_feature    1712..2491
                     /label=BBa_K116615
     misc_feature    1693..2491
                     /label=BBa_K116625
     misc_feature    885..2491
                     /label=BBa_K116634
     misc_feature    22..2491
                     /label=BBa_S04547
ORIGIN
        1 tcacacagga aagtactaga gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataatt actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      781 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      841 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcacac aggaaagtac
      901 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      961 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
     1021 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
     1081 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
     1141 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
     1201 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
     1261 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
     1321 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
     1381 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
     1441 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
     1501 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
     1561 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
     1621 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
     1681 tcactactag agtcacacag gaaagtacta gatgactata atgataaaaa aatcggattt
     1741 tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa
     1801 gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa
     1861 ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg
     1921 tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca
     1981 acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa
     2041 tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg aagctatata
     2101 taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga
     2161 gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt
     2221 attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc
     2281 agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     2341 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     2401 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     2461 tcaccttcgg gtgggccttt ctgcgtttat a
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