##gff-version 3
##sequence-region BBa_S04551 1 2491
BBa_S04551	.	engineered_region	22	2491	.	+	0	ID=BBa_S04547;Name=BBa_S04547
BBa_S04551	.	engineered_region	885	2491	.	+	0	ID=BBa_K116634;Name=BBa_K116634;Parent=BBa_S04547
BBa_S04551	.	engineered_region	1693	2491	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_S04551	.	ribosome_entry_site	1693	1705	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_S04551	.	ribosome_entry_site	1693	1705	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04551	.	ribosome_entry_site	1699	1702	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04551	.	engineered_region	1693	1705	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04551	.	engineered_region	1712	2491	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_S04551	.	terminator	2363	2491	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_S04551	.	terminator	2451	2491	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04551	.	stem_loop	2458	2477	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04551	.	polyA_site	2478	2491	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04551	.	stop_codon	2484	2484	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04551	.	engineered_region	2451	2491	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04551	.	terminator	2363	2442	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04551	.	engineered_region	2363	2442	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04551	.	stem_loop	2374	2417	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04551	.	CDS	1712	2329	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_S04551	.	engineered_tag	2330	2354	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04551	.	tag	2291	2322	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04551	.	engineered_region	1712	2329	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04551	.	CDS	1712	2290	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04551	.	engineered_region	885	1684	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_S04551	.	CDS	904	1659	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04551	.	engineered_tag	1660	1684	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04551	.	engineered_region	904	1659	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04551	.	engineered_region	904	905	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04551	.	point_mutation	1077	1077	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04551	.	point_mutation	1395	1395	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04551	.	CDS	904	1653	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04551	.	ribosome_entry_site	885	897	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04551	.	ribosome_entry_site	885	897	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04551	.	ribosome_entry_site	891	894	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04551	.	engineered_region	885	897	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04551	.	engineered_region	22	876	.	+	0	ID=BBa_K208016;Name=BBa_K208016;Parent=BBa_S04547
BBa_S04551	.	terminator	748	827	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208016
BBa_S04551	.	engineered_region	748	827	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04551	.	stem_loop	759	802	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04551	.	CDS	22	739	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K208016
BBa_S04551	.	CDS	22	739	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_S04551	.	point_mutation	22	22	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_S04551	.	terminator	836	876	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208016
BBa_S04551	.	stem_loop	843	862	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04551	.	polyA_site	863	876	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04551	.	stop_codon	869	869	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04551	.	engineered_region	836	876	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04551	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S04551	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04551	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04551	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_S04551
tcacacaggaaagtactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaataataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaa
attaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatt
tactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggca
atattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattgg
aatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactg
ggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatat
agatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaat
atagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagct
cttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaa
tacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataa
cactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgactataatgataaaaaaatcggat
tttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtggg
acttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatac
tgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgctt
ggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataa
ataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaata
tgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaa
attcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatg
ctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata