LOCUS       BBa_S04552              2489 bp    DNA     linear       01-Jun-2024
DEFINITION  B0033:S04547
ACCESSION   BBa_S04552
VERSION     BBa_S04552.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     CDS             20..737
                     /label=GFP fusion brick
     misc_feature    20..20
                     /label=deletion of TG
     CDS             20..737
                     /label=BBa_K125500
     stem_loop       757..800
                     /label=stem_loop
     misc_feature    746..825
                     /label=BBa_B0010
     terminator      746..825
                     /label=BBa_B0010
     stem_loop       841..860
                     /label=T7 TE
     misc_feature    867..867
                     /label=stop
     polyA_site      861..874
                     /label=polya
     misc_feature    834..874
                     /label=BBa_B0012
     terminator      834..874
                     /label=BBa_B0012
     misc_feature    20..874
                     /label=BBa_K208016
     RBS             883..895
                     /label=RBS-3\Weak
     RBS             889..892
                     /label=RBS
     misc_feature    883..895
                     /label=BBa_B0032
     RBS             883..895
                     /label=BBa_B0032
     misc_feature    902..903
                     /label=prefix
     misc_feature    1075..1075
                     /label=T
     misc_feature    1393..1393
                     /label=A
     CDS             902..1651
                     /label=luxR
     misc_feature    902..1657
                     /label=BBa_C0062
     misc_feature    1658..1682
                     /label=Help:Barcodes
     CDS             902..1657
                     /label=BBa_C0062
     misc_feature    883..1682
                     /label=BBa_K116616
     RBS             1691..1703
                     /label=RBS-3\Weak
     RBS             1697..1700
                     /label=RBS
     misc_feature    1691..1703
                     /label=BBa_B0032
     RBS             1691..1703
                     /label=BBa_B0032
     misc_feature    2289..2320
                     /label=LVA
     CDS             1710..2288
                     /label=luxI
     misc_feature    1710..2327
                     /label=BBa_C0061
     misc_feature    2328..2352
                     /label=Help:Barcodes
     CDS             1710..2327
                     /label=BBa_C0061
     stem_loop       2372..2415
                     /label=stem_loop
     misc_feature    2361..2440
                     /label=BBa_B0010
     terminator      2361..2440
                     /label=BBa_B0010
     stem_loop       2456..2475
                     /label=T7 TE
     misc_feature    2482..2482
                     /label=stop
     polyA_site      2476..2489
                     /label=polya
     misc_feature    2449..2489
                     /label=BBa_B0012
     terminator      2449..2489
                     /label=BBa_B0012
     terminator      2361..2489
                     /label=BBa_B0015
     misc_feature    1710..2489
                     /label=BBa_K116615
     misc_feature    1691..2489
                     /label=BBa_K116625
     misc_feature    883..2489
                     /label=BBa_K116634
     misc_feature    20..2489
                     /label=BBa_S04547
ORIGIN
        1 tcacacagga ctactagagc gtaaaggaga agaacttttc actggagttg tcccaattct
       61 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      121 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      181 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      241 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      301 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      361 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      421 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      481 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
      541 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      601 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
      661 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
      721 actatacaaa taataattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      781 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      841 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtcacacag gaaagtacta
      901 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      961 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
     1021 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
     1081 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
     1141 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
     1201 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
     1261 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
     1321 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
     1381 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
     1441 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
     1501 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
     1561 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
     1621 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
     1681 actactagag tcacacagga aagtactaga tgactataat gataaaaaaa tcggattttt
     1741 tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc
     1801 aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact
     1861 caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt
     1921 tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac
     1981 agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata
     2041 gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata
     2101 aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc
     2161 gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat
     2221 taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag
     2281 tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct
     2341 agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2401 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2461 accttcgggt gggcctttct gcgtttata
//