##gff-version 3
##sequence-region BBa_S04552 1 2489
BBa_S04552	.	ribosome_entry_site	1	11	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_S04552	.	engineered_region	1	11	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_S04552	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_S04552	.	ribosome_entry_site	1	11	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_S04552	.	engineered_region	20	2489	.	+	0	ID=BBa_S04547;Name=BBa_S04547
BBa_S04552	.	engineered_region	20	874	.	+	0	ID=BBa_K208016;Name=BBa_K208016;Parent=BBa_S04547
BBa_S04552	.	terminator	746	825	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208016
BBa_S04552	.	stem_loop	757	800	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04552	.	engineered_region	746	825	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04552	.	CDS	20	737	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K208016
BBa_S04552	.	CDS	20	737	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_S04552	.	point_mutation	20	20	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_S04552	.	terminator	834	874	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208016
BBa_S04552	.	polyA_site	861	874	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04552	.	stem_loop	841	860	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04552	.	stop_codon	867	867	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04552	.	engineered_region	834	874	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04552	.	engineered_region	883	2489	.	+	0	ID=BBa_K116634;Name=BBa_K116634;Parent=BBa_S04547
BBa_S04552	.	engineered_region	1691	2489	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_S04552	.	ribosome_entry_site	1691	1703	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_S04552	.	ribosome_entry_site	1691	1703	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04552	.	ribosome_entry_site	1697	1700	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04552	.	engineered_region	1691	1703	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04552	.	engineered_region	1710	2489	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_S04552	.	CDS	1710	2327	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_S04552	.	engineered_region	1710	2327	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04552	.	engineered_tag	2328	2352	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04552	.	CDS	1710	2288	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04552	.	tag	2289	2320	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04552	.	terminator	2361	2489	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_S04552	.	terminator	2449	2489	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04552	.	polyA_site	2476	2489	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04552	.	stem_loop	2456	2475	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04552	.	stop_codon	2482	2482	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04552	.	engineered_region	2449	2489	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04552	.	terminator	2361	2440	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04552	.	stem_loop	2372	2415	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04552	.	engineered_region	2361	2440	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04552	.	engineered_region	883	1682	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_S04552	.	CDS	902	1657	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04552	.	engineered_tag	1658	1682	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04552	.	engineered_region	902	1657	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04552	.	CDS	902	1651	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04552	.	point_mutation	1075	1075	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04552	.	engineered_region	902	903	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04552	.	point_mutation	1393	1393	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04552	.	ribosome_entry_site	883	895	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04552	.	ribosome_entry_site	883	895	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04552	.	ribosome_entry_site	889	892	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04552	.	engineered_region	883	895	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_S04552
tcacacaggactactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgat
gttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttattt
gcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagata
cccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc
aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaag
gtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcat
ggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagca
gaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctg
ccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatgg
catggatgaactatacaaataataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaat
taaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattattta
ctcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaat
attatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaa
tatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactggg
tttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatag
atagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatat
agcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctct
tgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaata
caacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataaca
ctgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgactataatgataaaaaaatcggattt
tttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggac
ttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactg
aaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttgg
tcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaat
aactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatg
taacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaat
tcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgct
gcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata