LOCUS       BBa_S04553              2490 bp    DNA     linear       05-Nov-2024
DEFINITION  B0034:S04547
ACCESSION   BBa_S04553
VERSION     BBa_S04553.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             21..738
                     /label=GFP fusion brick
     misc_feature    21..21
                     /label=deletion of TG
     CDS             21..738
                     /label=BBa_K125500
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     misc_feature    21..875
                     /label=BBa_K208016
     RBS             884..896
                     /label=RBS-3\Weak
     RBS             890..893
                     /label=RBS
     misc_feature    884..896
                     /label=BBa_B0032
     RBS             884..896
                     /label=BBa_B0032
     misc_feature    903..904
                     /label=prefix
     misc_feature    1076..1076
                     /label=T
     misc_feature    1394..1394
                     /label=A
     CDS             903..1652
                     /label=luxR
     misc_feature    903..1658
                     /label=BBa_C0062
     misc_feature    1659..1683
                     /label=Help:Barcodes
     CDS             903..1658
                     /label=BBa_C0062
     misc_feature    884..1683
                     /label=BBa_K116616
     RBS             1692..1704
                     /label=RBS-3\Weak
     RBS             1698..1701
                     /label=RBS
     misc_feature    1692..1704
                     /label=BBa_B0032
     RBS             1692..1704
                     /label=BBa_B0032
     misc_feature    2290..2321
                     /label=LVA
     CDS             1711..2289
                     /label=luxI
     misc_feature    1711..2328
                     /label=BBa_C0061
     misc_feature    2329..2353
                     /label=Help:Barcodes
     CDS             1711..2328
                     /label=BBa_C0061
     stem_loop       2373..2416
                     /label=stem_loop
     misc_feature    2362..2441
                     /label=BBa_B0010
     terminator      2362..2441
                     /label=BBa_B0010
     stem_loop       2457..2476
                     /label=T7 TE
     misc_feature    2483..2483
                     /label=stop
     polyA_site      2477..2490
                     /label=polya
     misc_feature    2450..2490
                     /label=BBa_B0012
     terminator      2450..2490
                     /label=BBa_B0012
     terminator      2362..2490
                     /label=BBa_B0015
     misc_feature    1711..2490
                     /label=BBa_K116615
     misc_feature    1692..2490
                     /label=BBa_K116625
     misc_feature    884..2490
                     /label=BBa_K116634
     misc_feature    21..2490
                     /label=BBa_S04547
ORIGIN
        1 aaagaggaga aatactagag cgtaaaggag aagaactttt cactggagtt gtcccaattc
       61 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      121 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      181 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
      241 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      301 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      361 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      421 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      481 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      541 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      601 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      661 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      721 aactatacaa ataataatta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtcacaca ggaaagtact
      901 agatgaaaaa cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta
      961 gaagcaataa tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat
     1021 attatttact cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag
     1081 ataattaccc taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc
     1141 ctatagtaga ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca
     1201 atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca
     1261 ctgggtttag tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac
     1321 attcagaaaa agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat
     1381 taattgttcc ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa
     1441 acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct
     1501 cttgggatat ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca
     1561 atgcgcaaat gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa
     1621 caggagcaat tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat
     1681 cactactaga gtcacacagg aaagtactag atgactataa tgataaaaaa atcggatttt
     1741 ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag
     1801 caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac
     1861 tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt
     1921 ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa
     1981 cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat
     2041 agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga agctatatat
     2101 aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag
     2161 cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta
     2221 ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca
     2281 gtcttaaatg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     2341 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2401 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2461 caccttcggg tgggcctttc tgcgtttata
//