##gff-version 3
##sequence-region BBa_S04553 1 2490
BBa_S04553	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S04553	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04553	.	engineered_region	21	2490	.	+	0	ID=BBa_S04547;Name=BBa_S04547
BBa_S04553	.	engineered_region	21	875	.	+	0	ID=BBa_K208016;Name=BBa_K208016;Parent=BBa_S04547
BBa_S04553	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208016
BBa_S04553	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04553	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04553	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208016
BBa_S04553	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04553	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04553	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04553	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04553	.	CDS	21	738	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K208016
BBa_S04553	.	CDS	21	738	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_S04553	.	point_mutation	21	21	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_S04553	.	engineered_region	884	2490	.	+	0	ID=BBa_K116634;Name=BBa_K116634;Parent=BBa_S04547
BBa_S04553	.	engineered_region	884	1683	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_S04553	.	CDS	903	1658	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04553	.	engineered_tag	1659	1683	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04553	.	engineered_region	903	904	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04553	.	engineered_region	903	1658	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04553	.	point_mutation	1394	1394	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04553	.	CDS	903	1652	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04553	.	point_mutation	1076	1076	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04553	.	ribosome_entry_site	884	896	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04553	.	engineered_region	884	896	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04553	.	ribosome_entry_site	884	896	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04553	.	ribosome_entry_site	890	893	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04553	.	engineered_region	1692	2490	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_S04553	.	ribosome_entry_site	1692	1704	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_S04553	.	engineered_region	1692	1704	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04553	.	ribosome_entry_site	1692	1704	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04553	.	ribosome_entry_site	1698	1701	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04553	.	engineered_region	1711	2490	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_S04553	.	CDS	1711	2328	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_S04553	.	engineered_region	1711	2328	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04553	.	tag	2290	2321	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04553	.	engineered_tag	2329	2353	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04553	.	CDS	1711	2289	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04553	.	terminator	2362	2490	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_S04553	.	terminator	2450	2490	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04553	.	stop_codon	2483	2483	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04553	.	polyA_site	2477	2490	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04553	.	stem_loop	2457	2476	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04553	.	engineered_region	2450	2490	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04553	.	terminator	2362	2441	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04553	.	stem_loop	2373	2416	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04553	.	engineered_region	2362	2441	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S04553
aaagaggagaaatactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtga
tgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatt
tgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagat
acccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattttt
caaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaa
ggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatca
tggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagc
agaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatct
gccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatg
gcatggatgaactatacaaataataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaa
ttaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattattt
actcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaa
tattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattgga
atatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgg
gtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatata
gatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaata
tagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctc
ttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaat
acaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataac
actgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgactataatgataaaaaaatcggatt
ttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtggga
cttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatact
gaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttg
gtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaa
taactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatat
gtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaa
ttcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgc
tgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata