LOCUS BBa_S04555 3563 bp DNA linear 05-Nov-2024 DEFINITION F2622:S04548 ACCESSION BBa_S04555 VERSION BBa_S04555.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 RBS 1071..1085 /label=RBS-1\Strong RBS 1078..1082 /label=RBS misc_feature 1071..1085 /label=BBa_B0030 RBS 1071..1085 /label=BBa_B0030 CDS 1094..1811 /label=GFP fusion brick misc_feature 1094..1094 /label=deletion of TG CDS 1094..1811 /label=BBa_K125500 stem_loop 1831..1874 /label=stem_loop misc_feature 1820..1899 /label=BBa_B0010 terminator 1820..1899 /label=BBa_B0010 stem_loop 1915..1934 /label=T7 TE misc_feature 1941..1941 /label=stop polyA_site 1935..1948 /label=polya misc_feature 1908..1948 /label=BBa_B0012 terminator 1908..1948 /label=BBa_B0012 misc_feature 1094..1948 /label=BBa_K208016 RBS 1957..1969 /label=RBS-3\Weak RBS 1963..1966 /label=RBS misc_feature 1957..1969 /label=BBa_B0032 RBS 1957..1969 /label=BBa_B0032 misc_feature 1976..1977 /label=prefix misc_feature 2149..2149 /label=T misc_feature 2467..2467 /label=A CDS 1976..2725 /label=luxR misc_feature 1976..2731 /label=BBa_C0062 misc_feature 2732..2756 /label=Help:Barcodes CDS 1976..2731 /label=BBa_C0062 misc_feature 1957..2756 /label=BBa_K116616 RBS 2765..2777 /label=RBS-3\Weak RBS 2771..2774 /label=RBS misc_feature 2765..2777 /label=BBa_B0032 RBS 2765..2777 /label=BBa_B0032 misc_feature 3363..3394 /label=LVA CDS 2784..3362 /label=luxI misc_feature 2784..3401 /label=BBa_C0061 misc_feature 3402..3426 /label=Help:Barcodes CDS 2784..3401 /label=BBa_C0061 stem_loop 3446..3489 /label=stem_loop misc_feature 3435..3514 /label=BBa_B0010 terminator 3435..3514 /label=BBa_B0010 stem_loop 3530..3549 /label=T7 TE misc_feature 3556..3556 /label=stop polyA_site 3550..3563 /label=polya misc_feature 3523..3563 /label=BBa_B0012 terminator 3523..3563 /label=BBa_B0012 terminator 3435..3563 /label=BBa_B0015 misc_feature 2784..3563 /label=BBa_K116615 misc_feature 2765..3563 /label=BBa_K116625 misc_feature 1957..3563 /label=BBa_K116634 misc_feature 1094..3563 /label=BBa_S04547 misc_feature 1071..3563 /label=BBa_S04548 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag attaaagagg 1081 agaaatacta gagcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga 1141 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 1201 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 1261 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca 1321 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac 1381 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga 1441 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct 1501 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca 1561 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca 1621 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 1681 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca 1741 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata 1801 caaataataa ttactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1861 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1921 ccttcgggtg ggcctttctg cgtttatata ctagagtcac acaggaaagt actagatgaa 1981 aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa 2041 taatgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt 2101 actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc tagataatta 2161 ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt 2221 agattattct aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt 2281 aaataaaaaa tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt 2341 tagtttccct attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga 2401 aaaagacaac tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt 2461 tccttctcta gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga 2521 tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga 2581 tatttcaaaa atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca 2641 aatgaaactc aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc 2701 aattgattgc ccatacttta aaaattaata acactgatag tgctagtgta gatcactact 2761 agagtcacac aggaaagtac tagatgacta taatgataaa aaaatcggat tttttggcaa 2821 ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac 2881 ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat aactcaaatg 2941 cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg cgtttattac 3001 ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt caacagagtg 3061 ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa 3121 agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata tataaacacg 3181 ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata gagcgatttt 3241 taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat gtattaggtg 3301 atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa 3361 atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta 3421 gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 3481 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 3541 gggtgggcct ttctgcgttt ata //