##gff-version 3
##sequence-region BBa_S04555 1 3563
BBa_S04555	.	engineered_region	1	1062	.	+	0	ID=BBa_F2622;Name=BBa_F2622
BBa_S04555	.	promoter	1008	1062	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2622
BBa_S04555	.	non_covalent_binding_site	1008	1027	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S04555	.	promoter	1049	1054	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S04555	.	start_codon	1060	1060	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S04555	.	promoter	1027	1032	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S04555	.	engineered_region	1008	1062	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S04555	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_F2622
BBa_S04555	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S04555	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S04555	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S04555	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S04555	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S04555	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2622
BBa_S04555	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04555	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04555	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2622
BBa_S04555	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04555	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2622
BBa_S04555	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04555	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04555	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04555	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04555	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2622
BBa_S04555	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04555	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04555	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04555	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04555	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04555	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04555	.	engineered_region	1071	3563	.	+	0	ID=BBa_S04548;Name=BBa_S04548
BBa_S04555	.	ribosome_entry_site	1071	1085	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_S04548
BBa_S04555	.	engineered_region	1071	1085	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04555	.	ribosome_entry_site	1071	1085	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04555	.	ribosome_entry_site	1078	1082	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04555	.	engineered_region	1094	3563	.	+	0	ID=BBa_S04547;Name=BBa_S04547;Parent=BBa_S04548
BBa_S04555	.	engineered_region	1957	3563	.	+	0	ID=BBa_K116634;Name=BBa_K116634;Parent=BBa_S04547
BBa_S04555	.	engineered_region	2765	3563	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_S04555	.	engineered_region	2784	3563	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_S04555	.	CDS	2784	3401	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_S04555	.	engineered_tag	3402	3426	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04555	.	engineered_region	2784	3401	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04555	.	CDS	2784	3362	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04555	.	tag	3363	3394	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04555	.	terminator	3435	3563	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_S04555	.	terminator	3523	3563	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04555	.	polyA_site	3550	3563	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04555	.	engineered_region	3523	3563	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04555	.	stop_codon	3556	3556	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04555	.	stem_loop	3530	3549	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04555	.	terminator	3435	3514	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04555	.	stem_loop	3446	3489	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04555	.	engineered_region	3435	3514	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04555	.	ribosome_entry_site	2765	2777	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_S04555	.	engineered_region	2765	2777	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04555	.	ribosome_entry_site	2765	2777	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04555	.	ribosome_entry_site	2771	2774	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04555	.	engineered_region	1957	2756	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_S04555	.	CDS	1976	2731	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04555	.	engineered_tag	2732	2756	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04555	.	CDS	1976	2725	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04555	.	point_mutation	2467	2467	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04555	.	engineered_region	1976	1977	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04555	.	engineered_region	1976	2731	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04555	.	point_mutation	2149	2149	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04555	.	ribosome_entry_site	1957	1969	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04555	.	engineered_region	1957	1969	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04555	.	ribosome_entry_site	1957	1969	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04555	.	ribosome_entry_site	1963	1966	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04555	.	engineered_region	1094	1948	.	+	0	ID=BBa_K208016;Name=BBa_K208016;Parent=BBa_S04547
BBa_S04555	.	terminator	1908	1948	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208016
BBa_S04555	.	polyA_site	1935	1948	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04555	.	engineered_region	1908	1948	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04555	.	stop_codon	1941	1941	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04555	.	stem_loop	1915	1934	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04555	.	CDS	1094	1811	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K208016
BBa_S04555	.	CDS	1094	1811	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_S04555	.	point_mutation	1094	1094	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_S04555	.	terminator	1820	1899	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208016
BBa_S04555	.	stem_loop	1831	1874	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04555	.	engineered_region	1820	1899	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S04555
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagagcgtaaaggagaag
aacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggcca
acacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttca
agagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctga
agtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattctt
ggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtta
acttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcga
tggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagac
cacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataattactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacacaggaaagtactag
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatc
aatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaa
atctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgat
cctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaat
ctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatgg
cttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacata
ccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacca
aaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtga
gcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagca
attttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagt
cacacaggaaagtactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata