LOCUS BBa_S04556 3562 bp DNA linear 05-Nov-2024 DEFINITION F2622:S04549 ACCESSION BBa_S04556 VERSION BBa_S04556.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1077..1080 /label=conserved RBS 1071..1084 /label=BBa_B0031 CDS 1093..1810 /label=GFP fusion brick misc_feature 1093..1093 /label=deletion of TG CDS 1093..1810 /label=BBa_K125500 stem_loop 1830..1873 /label=stem_loop misc_feature 1819..1898 /label=BBa_B0010 terminator 1819..1898 /label=BBa_B0010 stem_loop 1914..1933 /label=T7 TE misc_feature 1940..1940 /label=stop polyA_site 1934..1947 /label=polya misc_feature 1907..1947 /label=BBa_B0012 terminator 1907..1947 /label=BBa_B0012 misc_feature 1093..1947 /label=BBa_K208016 RBS 1956..1968 /label=RBS-3\Weak RBS 1962..1965 /label=RBS misc_feature 1956..1968 /label=BBa_B0032 RBS 1956..1968 /label=BBa_B0032 misc_feature 1975..1976 /label=prefix misc_feature 2148..2148 /label=T misc_feature 2466..2466 /label=A CDS 1975..2724 /label=luxR misc_feature 1975..2730 /label=BBa_C0062 misc_feature 2731..2755 /label=Help:Barcodes CDS 1975..2730 /label=BBa_C0062 misc_feature 1956..2755 /label=BBa_K116616 RBS 2764..2776 /label=RBS-3\Weak RBS 2770..2773 /label=RBS misc_feature 2764..2776 /label=BBa_B0032 RBS 2764..2776 /label=BBa_B0032 misc_feature 3362..3393 /label=LVA CDS 2783..3361 /label=luxI misc_feature 2783..3400 /label=BBa_C0061 misc_feature 3401..3425 /label=Help:Barcodes CDS 2783..3400 /label=BBa_C0061 stem_loop 3445..3488 /label=stem_loop misc_feature 3434..3513 /label=BBa_B0010 terminator 3434..3513 /label=BBa_B0010 stem_loop 3529..3548 /label=T7 TE misc_feature 3555..3555 /label=stop polyA_site 3549..3562 /label=polya misc_feature 3522..3562 /label=BBa_B0012 terminator 3522..3562 /label=BBa_B0012 terminator 3434..3562 /label=BBa_B0015 misc_feature 2783..3562 /label=BBa_K116615 misc_feature 2764..3562 /label=BBa_K116625 misc_feature 1956..3562 /label=BBa_K116634 misc_feature 1093..3562 /label=BBa_S04547 misc_feature 1071..3562 /label=BBa_S04549 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga 1081 aacctactag agcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 1141 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 1201 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 1261 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 1321 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 1381 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 1441 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 1501 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 1561 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 1621 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 1681 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 1741 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 1801 aaataataat tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1861 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1921 cttcgggtgg gcctttctgc gtttatatac tagagtcaca caggaaagta ctagatgaaa 1981 aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat 2041 aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta 2101 ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac 2161 cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta 2221 gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta 2281 aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt 2341 agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa 2401 aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt 2461 ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat 2521 ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat 2581 atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa 2641 atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca 2701 attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag atcactacta 2761 gagtcacaca ggaaagtact agatgactat aatgataaaa aaatcggatt ttttggcaat 2821 tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta agcaaagact 2881 tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata actcaaatgc 2941 agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc gtttattacc 3001 tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc 3061 tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa 3121 gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat ataaacacgc 3181 tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag agcgattttt 3241 aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg tattaggtga 3301 tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag cagtcttaaa 3361 tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag 3421 atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3481 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 3541 ggtgggcctt tctgcgttta ta //