LOCUS BBa_S04557 3561 bp DNA linear 05-Nov-2024 DEFINITION F2622:S04551 ACCESSION BBa_S04557 VERSION BBa_S04557.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 RBS 1071..1083 /label=RBS-3\Weak RBS 1077..1080 /label=RBS misc_feature 1071..1083 /label=BBa_B0032 RBS 1071..1083 /label=BBa_B0032 CDS 1092..1809 /label=GFP fusion brick misc_feature 1092..1092 /label=deletion of TG CDS 1092..1809 /label=BBa_K125500 stem_loop 1829..1872 /label=stem_loop misc_feature 1818..1897 /label=BBa_B0010 terminator 1818..1897 /label=BBa_B0010 stem_loop 1913..1932 /label=T7 TE misc_feature 1939..1939 /label=stop polyA_site 1933..1946 /label=polya misc_feature 1906..1946 /label=BBa_B0012 terminator 1906..1946 /label=BBa_B0012 misc_feature 1092..1946 /label=BBa_K208016 RBS 1955..1967 /label=RBS-3\Weak RBS 1961..1964 /label=RBS misc_feature 1955..1967 /label=BBa_B0032 RBS 1955..1967 /label=BBa_B0032 misc_feature 1974..1975 /label=prefix misc_feature 2147..2147 /label=T misc_feature 2465..2465 /label=A CDS 1974..2723 /label=luxR misc_feature 1974..2729 /label=BBa_C0062 misc_feature 2730..2754 /label=Help:Barcodes CDS 1974..2729 /label=BBa_C0062 misc_feature 1955..2754 /label=BBa_K116616 RBS 2763..2775 /label=RBS-3\Weak RBS 2769..2772 /label=RBS misc_feature 2763..2775 /label=BBa_B0032 RBS 2763..2775 /label=BBa_B0032 misc_feature 3361..3392 /label=LVA CDS 2782..3360 /label=luxI misc_feature 2782..3399 /label=BBa_C0061 misc_feature 3400..3424 /label=Help:Barcodes CDS 2782..3399 /label=BBa_C0061 stem_loop 3444..3487 /label=stem_loop misc_feature 3433..3512 /label=BBa_B0010 terminator 3433..3512 /label=BBa_B0010 stem_loop 3528..3547 /label=T7 TE misc_feature 3554..3554 /label=stop polyA_site 3548..3561 /label=polya misc_feature 3521..3561 /label=BBa_B0012 terminator 3521..3561 /label=BBa_B0012 terminator 3433..3561 /label=BBa_B0015 misc_feature 2782..3561 /label=BBa_K116615 misc_feature 2763..3561 /label=BBa_K116625 misc_feature 1955..3561 /label=BBa_K116634 misc_feature 1092..3561 /label=BBa_S04547 misc_feature 1071..3561 /label=BBa_S04551 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga 1081 aagtactaga gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat 1141 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa 1201 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc 1261 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata 1321 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta 1381 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata 1441 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg 1501 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa 1561 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac 1621 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca 1681 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca 1741 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca 1801 aataataatt actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1921 ttcgggtggg cctttctgcg tttatatact agagtcacac aggaaagtac tagatgaaaa 1981 acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata 2041 atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac 2101 tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc 2161 ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag 2221 attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa 2281 ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta 2341 gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa 2401 aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc 2461 cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt 2521 taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata 2581 tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa 2641 tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa 2701 ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag 2761 agtcacacag gaaagtacta gatgactata atgataaaaa aatcggattt tttggcaatt 2821 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 2881 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 2941 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 3001 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 3061 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 3121 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 3181 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 3241 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 3301 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 3361 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 3421 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 3481 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 3541 gtgggccttt ctgcgtttat a //