##gff-version 3
##sequence-region BBa_S04557 1 3561
BBa_S04557	.	engineered_region	1071	3561	.	+	0	ID=BBa_S04551;Name=BBa_S04551
BBa_S04557	.	engineered_region	1092	3561	.	+	0	ID=BBa_S04547;Name=BBa_S04547;Parent=BBa_S04551
BBa_S04557	.	engineered_region	1092	1946	.	+	0	ID=BBa_K208016;Name=BBa_K208016;Parent=BBa_S04547
BBa_S04557	.	terminator	1818	1897	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208016
BBa_S04557	.	stem_loop	1829	1872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04557	.	engineered_region	1818	1897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04557	.	terminator	1906	1946	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208016
BBa_S04557	.	stop_codon	1939	1939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04557	.	polyA_site	1933	1946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04557	.	stem_loop	1913	1932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04557	.	engineered_region	1906	1946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04557	.	CDS	1092	1809	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K208016
BBa_S04557	.	CDS	1092	1809	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_S04557	.	point_mutation	1092	1092	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_S04557	.	engineered_region	1955	3561	.	+	0	ID=BBa_K116634;Name=BBa_K116634;Parent=BBa_S04547
BBa_S04557	.	engineered_region	1955	2754	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_S04557	.	CDS	1974	2729	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04557	.	engineered_tag	2730	2754	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04557	.	engineered_region	1974	1975	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04557	.	engineered_region	1974	2729	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04557	.	point_mutation	2465	2465	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04557	.	CDS	1974	2723	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04557	.	point_mutation	2147	2147	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04557	.	ribosome_entry_site	1955	1967	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04557	.	engineered_region	1955	1967	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04557	.	ribosome_entry_site	1955	1967	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04557	.	ribosome_entry_site	1961	1964	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04557	.	engineered_region	2763	3561	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_S04557	.	ribosome_entry_site	2763	2775	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_S04557	.	engineered_region	2763	2775	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04557	.	ribosome_entry_site	2763	2775	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04557	.	ribosome_entry_site	2769	2772	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04557	.	engineered_region	2782	3561	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_S04557	.	CDS	2782	3399	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_S04557	.	engineered_region	2782	3399	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04557	.	tag	3361	3392	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04557	.	engineered_tag	3400	3424	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04557	.	CDS	2782	3360	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04557	.	terminator	3433	3561	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_S04557	.	terminator	3521	3561	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04557	.	stop_codon	3554	3554	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04557	.	polyA_site	3548	3561	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04557	.	stem_loop	3528	3547	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04557	.	engineered_region	3521	3561	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04557	.	terminator	3433	3512	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04557	.	stem_loop	3444	3487	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04557	.	engineered_region	3433	3512	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04557	.	ribosome_entry_site	1071	1083	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S04551
BBa_S04557	.	engineered_region	1071	1083	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04557	.	ribosome_entry_site	1071	1083	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04557	.	ribosome_entry_site	1077	1080	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04557	.	engineered_region	1	1062	.	+	0	ID=BBa_F2622;Name=BBa_F2622
BBa_S04557	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2622
BBa_S04557	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04557	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04557	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_F2622
BBa_S04557	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S04557	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S04557	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S04557	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S04557	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S04557	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2622
BBa_S04557	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04557	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2622
BBa_S04557	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04557	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04557	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04557	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04557	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2622
BBa_S04557	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04557	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04557	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04557	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04557	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04557	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04557	.	promoter	1008	1062	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2622
BBa_S04557	.	engineered_region	1008	1062	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S04557	.	non_covalent_binding_site	1008	1027	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S04557	.	start_codon	1060	1060	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S04557	.	promoter	1049	1054	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S04557	.	promoter	1027	1032	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
>BBa_S04557
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagagcgtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaag
tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgg
acacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaac
ttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatg
gccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacca
catggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataattactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacacaggaaagtactagat
gaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaa
tgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaat
ctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcc
tatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatct
ccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggct
tcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacc
attaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaa
agagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagc
gtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaat
tttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagtca
cacaggaaagtactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtatt
ctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagt
atgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctac
aacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtc
gaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttg
aagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattttt
aaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattg
tctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaat
aactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata