LOCUS BBa_S04558 3559 bp DNA linear 17-May-2024 DEFINITION F2622:S04552 ACCESSION BBa_S04558 VERSION BBa_S04558.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 RBS 1071..1081 /label=RBS-4\Weaker RBS 1077..1080 /label=RBS misc_feature 1071..1081 /label=BBa_B0033 RBS 1071..1081 /label=BBa_B0033 CDS 1090..1807 /label=GFP fusion brick misc_feature 1090..1090 /label=deletion of TG CDS 1090..1807 /label=BBa_K125500 stem_loop 1827..1870 /label=stem_loop misc_feature 1816..1895 /label=BBa_B0010 terminator 1816..1895 /label=BBa_B0010 stem_loop 1911..1930 /label=T7 TE misc_feature 1937..1937 /label=stop polyA_site 1931..1944 /label=polya misc_feature 1904..1944 /label=BBa_B0012 terminator 1904..1944 /label=BBa_B0012 misc_feature 1090..1944 /label=BBa_K208016 RBS 1953..1965 /label=RBS-3\Weak RBS 1959..1962 /label=RBS misc_feature 1953..1965 /label=BBa_B0032 RBS 1953..1965 /label=BBa_B0032 misc_feature 1972..1973 /label=prefix misc_feature 2145..2145 /label=T misc_feature 2463..2463 /label=A CDS 1972..2721 /label=luxR misc_feature 1972..2727 /label=BBa_C0062 misc_feature 2728..2752 /label=Help:Barcodes CDS 1972..2727 /label=BBa_C0062 misc_feature 1953..2752 /label=BBa_K116616 RBS 2761..2773 /label=RBS-3\Weak RBS 2767..2770 /label=RBS misc_feature 2761..2773 /label=BBa_B0032 RBS 2761..2773 /label=BBa_B0032 misc_feature 3359..3390 /label=LVA CDS 2780..3358 /label=luxI misc_feature 2780..3397 /label=BBa_C0061 misc_feature 3398..3422 /label=Help:Barcodes CDS 2780..3397 /label=BBa_C0061 stem_loop 3442..3485 /label=stem_loop misc_feature 3431..3510 /label=BBa_B0010 terminator 3431..3510 /label=BBa_B0010 stem_loop 3526..3545 /label=T7 TE misc_feature 3552..3552 /label=stop polyA_site 3546..3559 /label=polya misc_feature 3519..3559 /label=BBa_B0012 terminator 3519..3559 /label=BBa_B0012 terminator 3431..3559 /label=BBa_B0015 misc_feature 2780..3559 /label=BBa_K116615 misc_feature 2761..3559 /label=BBa_K116625 misc_feature 1953..3559 /label=BBa_K116634 misc_feature 1090..3559 /label=BBa_S04547 misc_feature 1071..3559 /label=BBa_S04552 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga 1081 ctactagagc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta 1141 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca 1201 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca 1261 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg 1321 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata 1381 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc 1441 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga 1501 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag 1561 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta 1621 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac 1681 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg 1741 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa 1801 taataattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1861 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1921 cgggtgggcc tttctgcgtt tatatactag agtcacacag gaaagtacta gatgaaaaac 1981 ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat 2041 gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc 2101 gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct 2161 aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat 2221 tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat 2281 aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac tgggtttagt 2341 ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca ttcagaaaaa 2401 gacaactata tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct 2461 tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta 2521 accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt 2581 tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg 2641 aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt 2701 gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc actactagag 2761 tcacacagga aagtactaga tgactataat gataaaaaaa tcggattttt tggcaattcc 2821 atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga 2881 gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga 2941 atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac 3001 aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc 3061 caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat 3121 aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt 3181 tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa 3241 gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac 3301 taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc 3361 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc 3421 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 3481 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 3541 gggcctttct gcgtttata //