BBa_S04562
type: ComponentDefinition
persistent identity: BBa S04562
display id: BBa_S04562
version: 1
was generated by: iGEM to SBOL conversion
description: S04561:K112808
created: 2011-06-21T11:00:00Z
modified: 2015-05-08T01:14:41Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 6080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Ebuzer Kalyoncu
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K112808
component: BBa_S04561
sequence annotation: BBa_S04561
sequence annotation: BBa_K112808
sequence: https://synbiohub.org/public/igem/BBa_S04562_sequence/1

BBa_K112808
type: Component
persistent identity: component2122467
display id: component2122467
version: 1
top level: BBa_S04562
definition: BBa_K112808
access: public

BBa_S04561
type: Component
persistent identity: component2122451
display id: component2122451
version: 1
top level: BBa_S04562
definition: BBa_S04561
access: public

BBa_S04561
type: SequenceAnnotation
persistent identity: annotation2122451
display id: annotation2122451
version: 1
top level: BBa_S04562
location: https://synbiohub.org/public/igem/BBa_S04562/annotation2122451/range2122451/1
component: BBa_S04561

https://synbiohub.org/public/igem/BBa_S04562/annotation2122451/range2122451/1
type: Range
persistent identity: range2122451
display id: range2122451
version: 1
top level: BBa_S04562
direction: direction/reverse
start: 1
end: 822
orientation: inline

BBa_K112808
type: SequenceAnnotation
persistent identity: annotation2122467
display id: annotation2122467
version: 1
top level: BBa_S04562
location: https://synbiohub.org/public/igem/BBa_S04562/annotation2122467/range2122467/1
component: BBa_K112808

https://synbiohub.org/public/igem/BBa_S04562/annotation2122467/range2122467/1
type: Range
persistent identity: range2122467
display id: range2122467
version: 1
top level: BBa_S04562
direction: direction/reverse
start: 831
end: 2615
orientation: inline

BBa_S04561
type: ComponentDefinition
persistent identity: BBa S04561
display id: BBa_S04561
version: 1
was generated by: iGEM to SBOL conversion
description: K115001:E0040
created: 2011-06-21T11:00:00Z
modified: 2015-05-08T01:14:41Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 6080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Ebuzer Kalyoncu
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0040
component: BBa_K115001
sequence annotation: BBa_K115001
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_S04561_sequence/1

BBa_E0040
type: Component
persistent identity: component2122441
display id: component2122441
version: 1
top level: BBa_S04561
definition: GFP
access: public

BBa_K115001
type: Component
persistent identity: component2122439
display id: component2122439
version: 1
top level: BBa_S04561
definition: BBa_K115001
access: public

BBa_K115001
type: SequenceAnnotation
persistent identity: annotation2122439
display id: annotation2122439
version: 1
top level: BBa_S04561
location: https://synbiohub.org/public/igem/BBa_S04561/annotation2122439/range2122439/1
component: BBa_K115001

https://synbiohub.org/public/igem/BBa_S04561/annotation2122439/range2122439/1
type: Range
persistent identity: range2122439
display id: range2122439
version: 1
top level: BBa_S04561
direction: direction/reverse
start: 1
end: 96
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2122441
display id: annotation2122441
version: 1
top level: BBa_S04561
location: https://synbiohub.org/public/igem/BBa_S04561/annotation2122441/range2122441/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S04561/annotation2122441/range2122441/1
type: Range
persistent identity: range2122441
display id: range2122441
version: 1
top level: BBa_S04561
direction: direction/reverse
start: 103
end: 822
orientation: inline

BBa_K112808
type: ComponentDefinition
persistent identity: BBa K112808
display id: BBa_K112808
version: 1
was generated by: iGEM to SBOL conversion
description: Enterobacteria phage T4 Lysis Device - no promoter
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Pubmed Accession Number: NC_000866 Enterobacteria phage T4
owned by: james
owned by: myers
mutable description: This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Various promoters can be installed with this device to control the lysis of bacteria. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: [Pcon].[Antiholin].[terminator] was assembled first, and then assembled with [T4 holin].[terminator]; Constitutive expression of antiholin is required before assembling holin.
star: true
creator: Jin Huh
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J23116
component: BBa_B0012
component: BBa_B0010
component: BBa_K112807
component: BBa_K112805
component: BBa_K112806
sequence annotation: BBa_K112805
sequence annotation: BBa_K112806
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23116
sequence annotation: BBa_K112807
sequence: https://synbiohub.org/public/igem/BBa_K112808_sequence/1

BBa_J23116
type: Component
persistent identity: component1999030
display id: component1999030
version: 1
top level: BBa_K112808
definition: BBa_J23116
access: public

BBa_B0012
type: Component
persistent identity: component1999026
display id: component1999026
version: 1
top level: BBa_K112808
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1999033
display id: component1999033
version: 1
top level: BBa_K112808
definition: BBa_B0010
access: public

BBa_K112807
type: Component
persistent identity: component1999032
display id: component1999032
version: 1
top level: BBa_K112808
definition: BBa_K112807
access: public

BBa_K112805
type: Component
persistent identity: component1999021
display id: component1999021
version: 1
top level: BBa_K112808
definition: BBa_K112805
access: public

BBa_B0010
type: Component
persistent identity: component1999024
display id: component1999024
version: 1
top level: BBa_K112808
definition: BBa_B0010
access: public

BBa_K112806
type: Component
persistent identity: component1999023
display id: component1999023
version: 1
top level: BBa_K112808
definition: BBa_K112806
access: public

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation1999021
display id: annotation1999021
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999021/range1999021/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K112808/annotation1999021/range1999021/1
type: Range
persistent identity: range1999021
display id: range1999021
version: 1
top level: BBa_K112808
direction: direction/reverse
start: 1
end: 676
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation1999023
display id: annotation1999023
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999023/range1999023/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K112808/annotation1999023/range1999023/1
type: Range
persistent identity: range1999023
display id: range1999023
version: 1
top level: BBa_K112808
direction: direction/reverse
start: 685
end: 1198
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1999024
display id: annotation1999024
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999024/range1999024/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K112808/annotation1999024/range1999024/1
type: Range
persistent identity: range1999024
display id: range1999024
version: 1
top level: BBa_K112808
direction: direction/forward
start: 1207
end: 1286
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1999026
display id: annotation1999026
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999026/range1999026/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K112808/annotation1999026/range1999026/1
type: Range
persistent identity: range1999026
display id: range1999026
version: 1
top level: BBa_K112808
direction: direction/forward
start: 1295
end: 1335
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation1999030
display id: annotation1999030
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999030/range1999030/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K112808/annotation1999030/range1999030/1
type: Range
persistent identity: range1999030
display id: range1999030
version: 1
top level: BBa_K112808
direction: direction/reverse
start: 1344
end: 1378
orientation: inline

BBa_K112807
type: SequenceAnnotation
persistent identity: annotation1999032
display id: annotation1999032
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999032/range1999032/1
component: BBa_K112807

https://synbiohub.org/public/igem/BBa_K112808/annotation1999032/range1999032/1
type: Range
persistent identity: range1999032
display id: range1999032
version: 1
top level: BBa_K112808
direction: direction/reverse
start: 1387
end: 1697
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1999033
display id: annotation1999033
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999033/range1999033/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K112808/annotation1999033/range1999033/1
type: Range
persistent identity: range1999033
display id: range1999033
version: 1
top level: BBa_K112808
direction: direction/forward
start: 1706
end: 1785
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_K115001
type: ComponentDefinition
persistent identity: BBa K115001
display id: BBa_K115001
version: 1
was generated by: iGEM to SBOL conversion
description: RNA thermometer (ROSE)
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
mutable provenance: This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein (prfA).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is designed as a temperature inducible RBS. Only designed so far.
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3007
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
star: true
creator: O.M.J.A. Stassen
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Predicted Stem Loop
sequence annotation: Predicted Stem Loop extending to startcodon
sequence annotation: Biobrick-Scar-adaptation
sequence annotation: SD
sequence: https://synbiohub.org/public/igem/BBa_K115001_sequence/1

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966926
display id: annotation1966926
version: 1
top level: BBa_K115001
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966926/range1966926/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K115001/annotation1966926/range1966926/1
type: Range
persistent identity: range1966926
display id: range1966926
version: 1
top level: BBa_K115001
direction: direction/forward
start: 1
end: 15
orientation: inline

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966927
display id: annotation1966927
version: 1
top level: BBa_K115001
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966927/range1966927/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K115001/annotation1966927/range1966927/1
type: Range
persistent identity: range1966927
display id: range1966927
version: 1
top level: BBa_K115001
direction: direction/forward
start: 16
end: 36
orientation: inline

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966928
display id: annotation1966928
version: 1
top level: BBa_K115001
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966928/range1966928/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115001/annotation1966928/range1966928/1
type: Range
persistent identity: range1966928
display id: range1966928
version: 1
top level: BBa_K115001
direction: direction/forward
start: 39
end: 71
orientation: inline

Predicted Stem Loop extending to startcodon
type: SequenceAnnotation
persistent identity: annotation1966929
display id: annotation1966929
version: 1
top level: BBa_K115001
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966929/range1966929/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115001/annotation1966929/range1966929/1
type: Range
persistent identity: range1966929
display id: range1966929
version: 1
top level: BBa_K115001
direction: direction/forward
start: 72
end: 96
orientation: inline

Biobrick-Scar-adaptation
type: SequenceAnnotation
persistent identity: annotation1966898
display id: annotation1966898
version: 1
top level: BBa_K115001
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966898/range1966898/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K115001/annotation1966898/range1966898/1
type: Range
persistent identity: range1966898
display id: range1966898
version: 1
top level: BBa_K115001
direction: direction/forward
start: 74
end: 79
orientation: inline

SD
type: SequenceAnnotation
persistent identity: annotation1966896
display id: annotation1966896
version: 1
top level: BBa_K115001
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966896/range1966896/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K115001/annotation1966896/range1966896/1
type: Range
persistent identity: range1966896
display id: range1966896
version: 1
top level: BBa_K115001
direction: direction/forward
start: 90
end: 95
orientation: inline

BBa_K112805
type: ComponentDefinition
persistent identity: BBa K112805
display id: BBa_K112805
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 holin]
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts
owned by: james
owned by: myers
mutable description: Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 holin
sequence: https://synbiohub.org/public/igem/BBa_K112805_sequence/1

T4 holin
type: SequenceAnnotation
persistent identity: annotation1999005
display id: annotation1999005
version: 1
top level: BBa_K112805
location: https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
type: Range
persistent identity: range1999005
display id: range1999005
version: 1
top level: BBa_K112805
direction: direction/forward
start: 17
end: 673
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 endolysin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: In stock
status: status/Available
bookmark: false
mutable notes: High expression of lysozyme only is toxic to bacteria.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
top level: BBa_K112806
direction: direction/forward
start: 17
end: 511
orientation: inline

BBa_K112807
type: ComponentDefinition
persistent identity: BBa K112807
display id: BBa_K112807
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 antiholin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts
owned by: james
owned by: myers
mutable description: Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria.
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 antiholin
sequence: https://synbiohub.org/public/igem/BBa_K112807_sequence/1

T4 antiholin
type: SequenceAnnotation
persistent identity: annotation1999019
display id: annotation1999019
version: 1
top level: BBa_K112807
location: https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
type: Range
persistent identity: range1999019
display id: range1999019
version: 1
top level: BBa_K112807
direction: direction/forward
start: 15
end: 308
orientation: inline

https://synbiohub.org/public/igem/BBa_S04562_sequence/1
type: Sequence
persistent identity: BBa S04562 sequence
display id: BBa_S04562_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggattactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04561_sequence/1
type: Sequence
persistent identity: BBa S04561 sequence
display id: BBa_S04561_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggattactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112808_sequence/1
type: Sequence
persistent identity: BBa K112808 sequence
display id: BBa_K112808_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K115001_sequence/1
type: Sequence
persistent identity: BBa K115001 sequence
display id: BBa_K115001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112805_sequence/1
type: Sequence
persistent identity: BBa K112805 sequence
display id: BBa_K112805_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112806_sequence/1
type: Sequence
persistent identity: BBa K112806 sequence
display id: BBa_K112806_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112807_sequence/1
type: Sequence
persistent identity: BBa K112807 sequence
display id: BBa_K112807_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z