LOCUS BBa_S04570 3423 bp DNA linear 05-Nov-2024 DEFINITION S04569:K116634 ACCESSION BBa_S04570 VERSION BBa_S04570.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 misc_feature 1..1082 /label=BBa_S04568 misc_feature 1089..1091 /label=Start CDS 1089..1808 /label=GFP Coding Region CDS 1089..1808 /label=BBa_K208000 misc_feature 1..1808 /label=BBa_S04569 RBS 1817..1829 /label=RBS-3\Weak RBS 1823..1826 /label=RBS misc_feature 1817..1829 /label=BBa_B0032 RBS 1817..1829 /label=BBa_B0032 misc_feature 1836..1837 /label=prefix misc_feature 2009..2009 /label=T misc_feature 2327..2327 /label=A CDS 1836..2585 /label=luxR misc_feature 1836..2591 /label=BBa_C0062 misc_feature 2592..2616 /label=Help:Barcodes CDS 1836..2591 /label=BBa_C0062 misc_feature 1817..2616 /label=BBa_K116616 RBS 2625..2637 /label=RBS-3\Weak RBS 2631..2634 /label=RBS misc_feature 2625..2637 /label=BBa_B0032 RBS 2625..2637 /label=BBa_B0032 misc_feature 3223..3254 /label=LVA CDS 2644..3222 /label=luxI misc_feature 2644..3261 /label=BBa_C0061 misc_feature 3262..3286 /label=Help:Barcodes CDS 2644..3261 /label=BBa_C0061 stem_loop 3306..3349 /label=stem_loop misc_feature 3295..3374 /label=BBa_B0010 terminator 3295..3374 /label=BBa_B0010 stem_loop 3390..3409 /label=T7 TE misc_feature 3416..3416 /label=stop polyA_site 3410..3423 /label=polya misc_feature 3383..3423 /label=BBa_B0012 terminator 3383..3423 /label=BBa_B0012 terminator 3295..3423 /label=BBa_B0015 misc_feature 2644..3423 /label=BBa_K116615 misc_feature 2625..3423 /label=BBa_K116625 misc_feature 1817..3423 /label=BBa_K116634 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat ggctagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg 1141 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg 1201 ctacatacgg aaagcttacc cttaaattta tttgcactac tggaaaacta cctgttccat 1261 ggccaacact tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc 1321 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca 1381 ctatatcttt caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg 1441 atacccttgt taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc 1501 tcggacacaa actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac 1561 aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggatccgttc 1621 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag 1681 acaaccatta cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc 1741 acatggtcct tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct 1801 acaaataata ctagagtcac acaggaaagt actagatgaa aaacataaat gccgacgaca 1861 catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct 1921 tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc 1981 attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat 2041 attatgatga cgctaattta ataaaatatg atcctatagt agattattct aactccaatc 2101 attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg 2161 taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg 2221 ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata 2281 gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt 2341 atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag 2401 aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt 2461 gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa 2521 accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta 2581 aaaattaata acactgatag tgctagtgta gatcactact agagtcacac aggaaagtac 2641 tagatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa 2701 ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta 2761 gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt 2821 gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg 2881 ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata 2941 gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt 3001 gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca 3061 gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct 3121 tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg 3181 tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac 3241 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagccaggc 3301 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3361 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3421 ata //