LOCUS       BBa_S04585              3480 bp    DNA     linear       05-Nov-2024
DEFINITION  I712074:pSB1AK8
ACCESSION   BBa_S04585
VERSION     BBa_S04585.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..75
                     /label=BioBrick suffix
     misc_feature    76..313
                     /label=terminator
     CDS             552..1166
                     /label=rep (pMB1)
     CDS             1388..2203
                     /label=kanR
     CDS             2358..3218
                     /label=ampR
     misc_feature    3459..3480
                     /label=BioBrick prefix
     misc_feature    55..3480
                     /label=pSB1AK8
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtactag
       61 tagcggccgc tgcagctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
      121 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      181 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      241 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
      301 ctggggatat gcaccggcaa aaaaacgggc aaggtgtcac caccctgccc tttttcttta
      361 aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg
      421 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      481 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      541 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      601 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      661 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      721 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      781 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      841 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      901 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      961 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     1021 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1081 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1141 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1201 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1261 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1321 gacagctcga gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca
     1381 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     1441 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     1501 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     1561 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     1621 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
     1681 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     1741 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     1801 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     1861 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     1921 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     1981 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     2041 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     2101 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     2161 atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     2221 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     2281 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     2341 tgaaggatca gctcgagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3361 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     3421 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
//