LOCUS       BBa_S04586              2122 bp    DNA     linear       16-Dec-2024
DEFINITION  J23119:pSB1A2
ACCESSION   BBa_S04586
VERSION     BBa_S04586.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    44..64
                     /label=Suffix
     primer_bind     122..141
                     /label=VR
     misc_feature    241..241
                     /label=ORI
     CDS             226..840
                     /label=rep (pMB1) (rev)
     CDS             1000..1860
                     /label=APr (rev)
     misc_feature    1895..1895
                     /label=pBLA (rev)
     primer_bind     1983..2002
                     /label=VF2
     stem_loop       2072..2091
                     /label=terminator
     misc_feature    2101..2122
                     /label=Prefix
     misc_feature    44..2122
                     /label=pSB1A2
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagtactagt agcggccgct
       61 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      661 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1981 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     2041 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
     2101 gaattcgcgg ccgcttctag ag
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