LOCUS       BBa_S04605              1587 bp    DNA     linear       23-May-2024
DEFINITION  S04603:E0240
ACCESSION   BBa_S04605
VERSION     BBa_S04605.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..703
                     /label=BBa_C0040
     misc_feature    1..703
                     /label=BBa_S04603
     RBS             712..724
                     /label=RBS-3\Weak
     RBS             718..721
                     /label=RBS
     misc_feature    712..724
                     /label=BBa_B0032
     RBS             712..724
                     /label=BBa_B0032
     CDS             731..1450
                     /label=GFP protein
     CDS             731..1450
                     /label=BBa_E0040
     stem_loop       1470..1513
                     /label=stem_loop
     misc_feature    1459..1538
                     /label=BBa_B0010
     terminator      1459..1538
                     /label=BBa_B0010
     stem_loop       1554..1573
                     /label=T7 TE
     misc_feature    1580..1580
                     /label=stop
     polyA_site      1574..1587
                     /label=polya
     misc_feature    1547..1587
                     /label=BBa_B0012
     terminator      1547..1587
                     /label=BBa_B0012
     misc_feature    712..1587
                     /label=BBa_E0240
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gtcacacagg
      721 aaagtactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      781 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      841 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      901 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      961 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     1021 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     1081 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     1141 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     1201 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     1261 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     1321 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1381 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1441 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1501 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1561 cttcgggtgg gcctttctgc gtttata
//