BBa_S04608 1 BBa_S04608 K531006:K531001 2011-09-20T11:00:00Z 2015-05-08T01:14:42Z false false _9_ 0 8840 9 It's complicated false false Alexander Aaring component2136594 1 BBa_K531001 component2136593 1 BBa_K531006 annotation2136594 1 BBa_K531001 range2136594 1 874 1233 annotation2136593 1 BBa_K531006 range2136593 1 1 865 BBa_K531001 1 BBa_K531001 <i>C. crescentus</i> S-layer RsaA C-term 2011-07-03T11:00:00Z 2015-05-08T01:12:36Z This comes from genomic ''Caulobacter'' CB15N. This is a secretion tag for secretion via a Type I secretion pathway in ''Caulobacter crescentus''. It should be appended to the C-terminal end of another protein to act as a secretion tag. false false _697_ 0 8840 9 In stock true Using the Registry's suggested primer combinations results in the formation of an in sequence stop codon between two coding regions. A one nucleotide change was necessary in both the prefix of this coding sequence and the suffix of the coding region to be added in front of this gene to ensure that their is no stop codon in sequence. false Alexander Aaring BBa_K531006 1 BBa_K531006 Optimized esp gene for <i>C. crescentus</i> 2011-07-25T11:00:00Z 2015-05-08T01:12:36Z Wild type esp sequence from Zhang,Y.Q., Ren,S.X., Li,H.L., Wang,Y.X., Fu,G., Yang,J., Qin,Z.Q., Miao,Y.G., Wang,W.Y., Chen,R.S., Shen,Y., Chen,Z., Yuan,Z.H., Zhao,G.P., Qu,D., Danchin,A. and Wen,Y.M. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49 (6), 1577-1593 (2003). Optimized sequence is based on the wild type sequence. The wild Type esp is secreted by S. epidermidis, a bacteria that has low G/C content in its genome. It is then optimized to give a high G/C content without changing any amino acids sequence. false false _697_ 0 8860 9 It's complicated false DNA codons are optimized for <i>C. crescentus</i> expression. false Qimeng Gao BBa_S04608_sequence 1 tgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagtactagaggatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa BBa_K531006_sequence 1 tgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccag BBa_K531001_sequence 1 gatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z