LOCUS BBa_S04623 3420 bp DNA linear 22-Dec-2024 DEFINITION K561000:K561002 ACCESSION BBa_S04623 VERSION BBa_S04623.1 FEATURES Location/Qualifiers promoter 1..135 /label=vgb promoter promoter 1..135 /label=BBa_K561001 RBS 144..156 /label=RBS-3\Weak RBS 150..153 /label=RBS misc_feature 144..156 /label=BBa_B0032 RBS 144..156 /label=BBa_B0032 CDS 163..885 /label=BBa_E0030 misc_feature 144..885 /label=BBa_I15017 misc_feature 898..901 /label=conserved RBS 894..905 /label=BBa_B0034 CDS 915..1531 /label=tetR misc_feature 1532..1565 /label=SsrA misc_feature 1572..1596 /label=Help:Barcodes CDS 912..1571 /label=BBa_C0040 stem_loop 1616..1659 /label=stem_loop misc_feature 1605..1684 /label=BBa_B0010 terminator 1605..1684 /label=BBa_B0010 stem_loop 1700..1719 /label=T7 TE misc_feature 1726..1726 /label=stop polyA_site 1720..1733 /label=polya misc_feature 1693..1733 /label=BBa_B0012 terminator 1693..1733 /label=BBa_B0012 misc_feature 894..1733 /label=BBa_P0440 misc_feature 1..1733 /label=BBa_K561000 promoter 1742..1840 /label=BBa_K387003 misc_feature 1853..1856 /label=conserved RBS 1849..1860 /label=BBa_B0034 CDS 1867..2541 /label=mrfp1 misc_feature 2548..2572 /label=Help:Barcodes CDS 1867..2572 /label=BBa_E1010 misc_feature 1849..2572 /label=BBa_K093005 misc_feature 2585..2588 /label=conserved RBS 2581..2592 /label=BBa_B0034 CDS 2602..3218 /label=tetR misc_feature 3219..3252 /label=SsrA misc_feature 3259..3283 /label=Help:Barcodes CDS 2599..3258 /label=BBa_C0040 stem_loop 3303..3346 /label=stem_loop misc_feature 3292..3371 /label=BBa_B0010 terminator 3292..3371 /label=BBa_B0010 stem_loop 3387..3406 /label=T7 TE misc_feature 3413..3413 /label=stop polyA_site 3407..3420 /label=polya misc_feature 3380..3420 /label=BBa_B0012 terminator 3380..3420 /label=BBa_B0012 misc_feature 2581..3420 /label=BBa_P0440 misc_feature 1742..3420 /label=BBa_K561002 ORIGIN 1 acaggacgct ggggttaaaa gtatttgagt tttgatgtgg attaagtttt aagaggcaat 61 aaagattata ataagtgctg ctacaccata ctgatgtatg gcaaaaccat aataatgaac 121 ttaaggaaga ccctctacta gagtcacaca ggaaagtact agatggtgag caagggcgag 181 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 241 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 301 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc 361 tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga cttcttcaag 421 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 481 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 541 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 601 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc 661 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 721 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc 781 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 841 gccgccggga tcactctcgg catggacgag ctgtacaagt aataatacta gagaaagagg 901 agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc 961 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag 1021 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca 1081 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag 1141 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag 1201 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat 1261 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg 1321 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag 1381 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat 1441 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat 1501 tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag 1561 tagcttaata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa 1621 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 1681 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 1741 gtgaataaat agtgcccgta atatcaggga atgaccccac ataaaatgtg gcataaaaga 1801 tgcatactgt agtcgagagc gcgtatgcgt gatttgatta tactagagaa agaggagaaa 1861 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg 1921 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac 1981 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg 2041 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac 2101 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac 2161 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc 2221 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa 2281 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa 2341 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa 2401 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa 2461 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa 2521 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag 2581 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 2641 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 2701 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 2761 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 2821 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 2881 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 2941 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 3001 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 3061 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 3121 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 3181 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 3241 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 3301 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3361 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //